About: Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus

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About: Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus
Creator	Baumgärtner, Wolfgang Pringproa, Kidsadagon Praphet, Reunkeaw Pruksananonda, Kamthorn Rungsiwiwut, Ruttachuk Tantilertcharoen, Rachod Thanawongnuwech, Roongroje Chan, Renee
Source	PMC
abstract	Central nervous system (CNS) dysfunction caused by neurovirulent influenza viruses is a dreaded complication of infection, and may play a role in some neurodegenerative conditions, such as Parkinson-like diseases and encephalitis lethargica. Although CNS infection by highly pathogenic H5N1 virus has been demonstrated, it is unknown whether H5N1 infects neural progenitor cells, nor whether such infection plays a role in the neuroinflammation and neurodegeneration. To pursue this question, we infected human neural progenitor cells (hNPCs) differentiated from human embryonic stem cells in vitro with H5N1 virus, and studied the resulting cytopathology, cytokine expression, and genes involved in the differentiation. Human embryonic stem cells (BG01) were maintained and differentiated into the neural progenitors, and then infected by H5N1 virus (A/Chicken/Thailand/CUK2/04) at a multiplicity of infection of 1. At 6, 24, 48, and 72 hours post-infection (hpi), cytopathic effects were observed. Then cells were characterized by immunofluorescence and electron microscopy, supernatants quantified for virus titers, and sampled cells studied for candidate genes.The hNPCs were susceptible to H5N1 virus infection as determined by morphological observation and immunofluorescence. The infection was characterized by a significant up-regulation of TNF-α gene expression, while expressions of IFN-α2, IFN-β1, IFN-γ and IL-6 remained unchanged compared to mock-infected controls. Moreover, H5N1 infection did not appear to alter expression of neuronal and astrocytic markers of hNPCs, such as β-III tubulin and GFAP, respectively. The results indicate that hNPCs support H5N1 virus infection and may play a role in the neuroinflammation during acute viral encephalitis.
has issue date	2015-08-14(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0135850
bibo:pmid	26274828
has license	cc-by
sha1sum (hex)	cb763a61411514366d16bb2571461b6df1361517
schema:url	https://doi.org/10.1371/journal.pone.0135850
resource representing a document's title	Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus
has PubMed Central identifier	PMC4537284
has PubMed identifier	26274828
schema:publication	PLoS One
resource representing a document's body	covid:cb763a61411514366d16bb2571461b6df1361517#body_text
is schema:about of	named entity 'embryonic stem cells' named entity 'multiplicity' named entity 'involved' named entity 'caused' named entity 'titers' named entity 'Influenza' named entity 'INOCULATION' named entity 'NEURAL' covid:arg/cb763a61411514366d16bb2571461b6df1361517 named entity '72 HOURS' named entity 'INFECTION' named entity 'COMPARED' named entity 'DID' named entity 'EXPRESSIONS' named entity 'INFECTED' named entity 'MAINTAINED' named entity 'SIGNIFICANT' named entity 'IL-6' named entity 'RESULTS' named entity 'ROLE' named entity 'NEUROINFLAMMATION' named entity 'SAMPLED' named entity 'ASTROCYTIC' named entity 'H5N1 VIRUS' named entity 'DIFFERENTIATED FROM' named entity 'HIGHLY' named entity '28A' named entity 'PROGENITOR CELLS' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'GENES' named entity 'CAUSED BY' named entity 'RESULTING' named entity 'CONDITIONS' named entity 'CNS INFECTION' named entity 'H5N1' named entity 'PARKINSON' named entity 'NEURAL' named entity 'INVOLVED' named entity 'UNKNOWN' named entity 'GFAP' named entity 'OBSERVED' named entity 'CHARACTERIZED' named entity 'IMMUNOFLUORESCENCE' named entity 'B21' named entity 'DISEASES' named entity 'NEURAL PROGENITOR' named entity 'MULTIPLICITY' named entity 'VIRUS INFECTION' named entity 'GENE EXPRESSION' named entity 'DEMONSTRATED' named entity 'COMPLICATION' named entity 'H5N1 INFLUENZA' named entity 'TUBULIN' named entity 'LIKE' named entity 'IS A' named entity 'POST' named entity 'DIFFERENTIATED' named entity 'DIFFERENTIATION' named entity 'DERIVED' named entity 'UP-REGULATION' named entity 'CYTOPATHIC EFFECTS' named entity 'EMBRYONIC' named entity 'PATHOGENIC' named entity 'SUSCEPTIBLE' named entity 'INFECTED HUMAN' named entity 'CANDIDATE GENES' named entity 'DETERMINED BY' named entity 'B12' named entity 'HUMAN EMBRYONIC STEM CELLS' named entity 'INFLUENZA VIRUSES' named entity 'MARKERS' named entity 'VIRUS' named entity 'INFECTS' named entity 'ENCEPHALITIS LETHARGICA'

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