About: Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus

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About: Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus
Creator	Lu, Jiaqi Yuan, Shishan Zheng, Haihong Lv, Jian Sun, Zhi Yu, Dandan
Source	Elsevier; Medline; PMC
abstract	Abstract The overlapping genomic regions coding for structural proteins of porcine reproductive and respiratory syndrome virus (PRRSV) poses problems for molecular dissection of the virus replication process. We constructed five mutant full-length cDNA clones with the overlapping regions unwound and 1 to 3 restriction sites inserted between two adjacent ORFs (ORF1/2, ORF4/5, ORF5/6, ORF 6/7 and ORF7/3′ UTR), which generated the recombinant viruses. Our findings demonstrated that 1) the overlapping structural protein ORFs can be physically separated, and is dispensable for virus viability; 2) such ORF separations did not interrupt the subgenomic RNA synthesis; 3) the plaque morphology, growth kinetics, and antigenicity of these mutant viruses were virtually indistinguishable from those of the parental virus in cultured cells; and 4) these mutant viruses remained genetic stable in vitro. This study lays a foundation for further molecular dissection of PRRSV replication process, and development of genetically tagged vaccines against PRRS.
has issue date	2009-01-05(xsd:dateTime)
bibo:doi	10.1016/j.virol.2008.09.013
bibo:pmid	18977502
has license	els-covid
sha1sum (hex)	cafc9173f5e4cf38ffa99b4799dd44c71c4f0853
schema:url	https://doi.org/10.1016/j.virol.2008.09.013
resource representing a document's title	Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus
has PubMed Central identifier	PMC7172853
has PubMed identifier	18977502
schema:publication	Virology
resource representing a document's body	covid:cafc9173f5e4cf38ffa99b4799dd44c71c4f0853#body_text
is schema:about of	named entity 'genetically' named entity 'cDNA clones' named entity 'porcine' named entity 'This' named entity 'poses' named entity 'reproductive' named entity 'virtually' named entity 'type II' named entity 'respiratory' named entity 'CLONES' named entity '80%' named entity 'RNA SYNTHESIS' named entity 'ORF7' named entity 'GROWTH KINETICS' named entity 'mutant' named entity 'ORF' named entity 'genomic' named entity 'genetic' named entity 'ORFs' named entity 'coding' named entity 'virus' named entity 'respiratory' named entity 'remained' named entity 'cultured cells' named entity 'replication process' named entity 'porcine reproductive and respiratory syndrome virus' named entity 'viruses' named entity 'viruses' named entity 'restriction sites' named entity 'coding regions' named entity 'porcine reproductive and respiratory syndrome virus' named entity 'vaccine' named entity 'subgenomic RNAs' named entity 'swine' named entity 'PRRSV' named entity 'ORFs' named entity 'PCR' named entity 'C-terminus' named entity 'PRRSV' named entity 'RNA transcripts' named entity 'China' named entity 'transfection' named entity 'vaccine' named entity 'PRRSV' named entity 'Germany' named entity 'transcription regulation' named entity 'Nsp2' named entity 'UV light' named entity 'EAV' named entity 'structural protein' named entity 'PRRS' named entity 'secondary structure' named entity 'wild-type virus' named entity 'North American' named entity 'genetic manipulations' named entity 'secondary structure' named entity 'plaque assays' named entity 'ORFs' named entity 'clones' named entity 'United States' named entity 'avian influenza' named entity 'RNA molecules' named entity 'morphology' named entity 'Genetic manipulation' named entity 'cDNA clones' named entity 'transcription' named entity 'nucleotide sequence' named entity 'reverse genetic' named entity 'Germany' named entity '37°C' named entity 'Invitrogen' named entity 'ORF' named entity 'coronaviruses' named entity 'virus' named entity 'similar manner' named entity 'PRRSV'

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