About: Nasopharyngeal bacterial load as a marker for rapid and easy diagnosis of invasive pneumococcal disease in children from Mozambique

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About: Nasopharyngeal bacterial load as a marker for rapid and easy diagnosis of invasive pneumococcal disease in children from Mozambique Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Nasopharyngeal bacterial load as a marker for rapid and easy diagnosis of invasive pneumococcal disease in children from Mozambique
Creator	Brotons, Pedro Muñoz-Almagro, Carmen Bassat, Quique Acacio, Sozinho Andres-Franch, Maria Balcells, Reyes Henares, Desiree Lanaspa, Miguel Madrid, Lola Perez-Arguello, Amaresh Valero-Rello, Ana Marcos, Maria
source	Medline; PMC
abstract	BACKGROUND: Current diagnostic methods for detection of Streptococcus pneumoniae in children with suspected invasive pneumococcal disease have limitations of accuracy, timeliness, and patient convenience. This study aimed to determine the performance of pneumococcal load quantified with a real-time polymerase-chain reaction in nasopharyngeal samples to diagnose invasive pneumococcal disease in children. METHODS: Matched case-control study of patients <5 years of age with invasive pneumococcal disease admitted to the Manhiça District Hospital (Mozambique) and asymptomatic controls recruited in different periods between 2006 and 2014. Cases were confirmed by a positive bacterial culture for S. pneumoniae in blood or cerebrospinal fluid. Nasopharyngeal aspirates were collected from cases and controls and pneumococcal density was quantified by lytA real-time polymerase-chain reaction. RESULTS: Thirty cases (median age 12.8 months) and sixty controls (median age 11.7 months) were enrolled and 70% of them were male. Nasopharyngeal pneumococcal carriage was high in both groups: 28/30 (93.3%) for cases vs. 53/60 (88.3%) for controls (p = 0.71). Mean nasopharyngeal pneumococcal load was identified as a marker for invasive pneumococcal disease (7.0 log(10) copies/mL in cases vs. 5.8 log(10) copies/mL in controls, p<0.001) and showed good discriminatory power (AUC-ROC: 82.1%, 95% CI 72.5%-91.8%). A colonization density of 6.5 log(10) copies/mL was determined as the optimal cut-off value to distinguish cases from controls (sensitivity 75.0%, specificity 73.6%). CONCLUSION: Use of non-invasive nasopharyngeal aspirates coupled with rapid and accurate quantification of pneumococcal load by real-time polymerase chain reaction has the potential to become a useful surrogate marker for early diagnosis of invasive pneumococcal disease in children.
has issue date	2017-09-14(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0184762
bibo:pmid	28910402
has license	cc-by
sha1sum (hex)	ca0eb068e5fa4d0bdb05c7d5d6a2a0e0fbd36e40
schema:url	https://doi.org/10.1371/journal.pone.0184762
resource representing a document's title	Nasopharyngeal bacterial load as a marker for rapid and easy diagnosis of invasive pneumococcal disease in children from Mozambique
has PubMed Central identifier	PMC5599037
has PubMed identifier	28910402
schema:publication	PLoS One
resource representing a document's body	covid:ca0eb068e5fa4d0bdb05c7d5d6a2a0e0fbd36e40#body_text
is schema:about of	named entity 'DIAGNOSE' named entity 'PERFORMANCE' named entity 'NASOPHARYNGEAL' named entity 'PNEUMOCOCCAL' named entity 'CHAIN REACTION' named entity 'PATIENT' named entity 'Streptococcus' named entity 'MARKER' named entity 'LOAD' named entity 'SAMPLES' named entity 'METHODS' named entity 'SUSPECTED' named entity 'INVASIVE PNEUMOCOCCAL DISEASE' named entity 'CURRENT' named entity 'STREPTOCOCCUS PNEUMONIAE' named entity 'DETECTION' named entity 'REAL-TIME' named entity 'LIMITATIONS' named entity 'DIAGNOSIS' named entity 'BACTERIAL LOAD' named entity 'INVASIVE PNEUMOCOCCAL DISEASE' named entity 'MOZAMBIQUE' named entity 'NASOPHARYNGEAL' named entity 'RAPID' named entity 'EASY' named entity 'STUDY' named entity 'HAVE' named entity 'DIAGNOSTIC' named entity 'CHILDREN' named entity 'ACCURACY' named entity 'POLYMERASE' named entity 'CONVENIENCE' named entity 'TIMELINESS' named entity 'CHILDREN' named entity 'DETERMINE' covid:arg/ca0eb068e5fa4d0bdb05c7d5d6a2a0e0fbd36e40 named entity 'limitations' named entity 'This' named entity 'samples' named entity 'invasive pneumococcal disease' named entity 'Streptococcus pneumoniae' named entity 'nasopharyngeal' named entity 'pneumococcal' named entity 'Mozambique' named entity 'invasive pneumococcal disease' named entity 'Spain' named entity 'confounding' named entity 'weight-for-age' named entity 'risk factors' named entity 'Branchburg' named entity 'urine' named entity 'pneumococcal' named entity 'XLS' named entity 'PCR' named entity 'genomic DNA' named entity 'holistic approach' named entity 'Mozambique' named entity 'pneumococcal pneumonia' named entity 'CFU' named entity 'pneumococcal' named entity 'adenovirus' named entity 'influenza' named entity 'asymptomatic' named entity '2.6' named entity 'Statistical significance' named entity 'body weight' named entity 'pneumococcal' named entity 'meningitis' named entity 'pediatric' named entity 'sample size'

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