About: Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection

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About: Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection
Creator	Chen, Hongyan Ge, Junwei Cui, Xingyang Shi, Yunjia Zhao, Lili Wei, Chengwei Wen, Shanshan Xia, Shuang
source	Medline; PMC
abstract	BACKGROUND: Mink circovirus (MiCV) is a newly discovered pathogen associated with mink diarrhea. The prevalence and economic importance of this virus remain poorly understood, and no specific serological assay has been developed for the diagnosis of MiCV infection. RESULTS: In this study, a recombinant capsid protein antigen expressed in Escherichia coli was utilized to establish an indirect enzyme-linked immunosorbent assay (iELISA). Results revealed that the assay had no cross-reactivity with other related pathogens, and the respective sensitivity and specificity of the proposed iELISA were 92.31% and 91.67% compared with those obtained of Western blot on 138 serum samples from minks. The correlation coefficient between iELISA and Western blot was 0.838 (p > 0.05). iELISA was applied to detect MiCV antibodies in 683 clinical serum samples from different farms from the major mink industry province in China, and 21 of 24 farms with 163 of 683 (23.87%) individuals were tested positive for MiCV antibodies. The positive rates of each of the 21 flocks ranged from 2.33% to 73.68%. CONCLUSIONS: These results indicated that iELISA was a sensitive and specific method suitable for the large-scale detection of MiCV infections in mink. This study provided an effective method for the serological diagnosis and positive rate investigation of MiCV infection.
has issue date	2018-01-26(xsd:dateTime)
bibo:doi	10.1186/s12917-018-1337-z
bibo:pmid	29373971
has license	cc-by
sha1sum (hex)	c988426bbcd0eb076538fb7f7c60db4762ae24bf
schema:url	https://doi.org/10.1186/s12917-018-1337-z
resource representing a document's title	Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection
has PubMed Central identifier	PMC5787310
has PubMed identifier	29373971
schema:publication	BMC Vet Res
resource representing a document's body	covid:c988426bbcd0eb076538fb7f7c60db4762ae24bf#body_text
is schema:about of	named entity 'circovirus' named entity 'remain' named entity 'capsid' named entity 'INFECTIONS' named entity 'INDIRECT' named entity 'COMPARED' named entity 'MINKS' named entity 'DIAGNOSIS' named entity 'PATHOGEN' named entity 'OBTAINED' named entity 'POORLY' named entity 'CONCLUSIONS' named entity 'ANTIBODIES' named entity 'antibodies' named entity 'study' named entity 'China' named entity 'infection' named entity 'revealed' named entity 'virus' named entity 'Development' named entity 'MiCV' named entity 'diarrhea' named entity 'MiCV' named entity 'serological' named entity 'China' named entity 'antibodies' named entity 'infection' named entity 'correlation coefficient' named entity 'circovirus' named entity 'infection' named entity 'sensitivity and specificity' named entity 'antigen' named entity 'antibodies' named entity 'cross-reactivity' named entity 'samples' named entity 'infection' named entity 'MiCV' named entity 'sensitive and specific' named entity 'DIFFERENT' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'serum samples' named entity 'assay' named entity 'MiCV' named entity 'MiCV' named entity 'capsid protein' named entity 'CROSS' named entity 'SAMPLES' named entity 'INFECTION' named entity '163' named entity 'SERUM' named entity 'PROPOSED' named entity 'POSITIVE FOR' named entity 'CLINICAL' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'ECONOMIC IMPORTANCE' named entity 'CAPSID PROTEIN' named entity 'ESCHERICHIA COLI' named entity 'MAJOR' named entity 'MINK' named entity 'WESTERN BLOT' named entity 'infection' named entity 'SEROLOGICAL DIAGNOSIS' named entity 'APPLIED' named entity 'RESULTS' named entity 'INVESTIGATION' named entity 'RECOMBINANT' named entity 'PROVINCE' named entity 'DETECTION' named entity 'RELATED' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'RECOMBINANT' named entity 'capsid protein' named entity 'enzyme-linked immunosorbent assay' named entity 'MiCV'

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