About: Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2

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About: Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2 Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2
Creator	Munir, Muhammad Branavan, Manoharanehru Rohaim, Mohammed Bayoumi, Mahmoud Poirier, Al-Natour, Mohammad Bates, Michelle Botham, Judy Boyce, Joe Brown, Amy Burkhart, Peter Chaudhry, Nouman Clayton, Emily Fernando, Anil Hacking, Wendy Khalifa, Manar Ragione, Roberto Sahin, Irem Sodi, Ravinder Tharmakulasingam, Mukunthan Vilela, Julianne Wilkinson, Hayley
source	MedRxiv
abstract	Until vaccines and effective therapeutics become available, the practical way to transit safely out of the current lockdown may include the implementation of an effective testing, tracing and tracking system. However, this requires a reliable and clinically validated diagnostic platform for the sensitive and specific identification of SARS-CoV-2. Here, we report on the development of a de novo, high-resolution and comparative genomics guided reverse-transcribed loop-mediated isothermal amplification (LAMP) assay. To further enhance the assay performance and to remove any subjectivity associated with operator interpretation of result, we engineered a novel hand-held smart diagnostic device. The robust diagnostic device was further furnished with automated image acquisition and processing algorithms, and the collated data was processed through artificial intelligence (AI) pipelines to further reduce the assay run time and the subjectivity of the colorimetric LAMP detection. This advanced AI algorithm-implemented LAMP (ai-LAMP) assay, targeting the RNA-dependent RNA polymerase gene, showed high analytical sensitivity and specificity for SARS-CoV-2. A total of ~200 coronavirus disease (CoVID-19)-suspected patient samples were tested using the platform and it was shown to be reliable, highly specific and significantly more sensitive than the current gold standard qRT-PCR. The system could provide an efficient and cost-effective platform to detect SARS-CoV-2 in resource-limited laboratories.
has issue date	2020-07-10(xsd:dateTime)
bibo:doi	10.1101/2020.07.08.20148999
has license	medrxiv
sha1sum (hex)	c944f7cb14fe5b4de0ac9e06f7fb2ea6276df046
schema:url	https://doi.org/10.1101/2020.07.08.20148999
resource representing a document's title	Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2
resource representing a document's body	covid:c944f7cb14fe5b4de0ac9e06f7fb2ea6276df046#body_text
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