About: Treating Influenza and SARS-CoV-2 via mRNA-encoded Cas13a

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Treating Influenza and SARS-CoV-2 via mRNA-encoded Cas13a Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Treating Influenza and SARS-CoV-2 via mRNA-encoded Cas13a
Creator	Michel, Frank Vanover, Daryll Santangelo, Philip Blanchard, Emmeline Hogan, Robert Lafontaine, Eric Tiwari, Pooja Subhash Bawage, Swapnil Beyersdorf, Jared Bruno, Nicholas Finn, M Hincapie, Robert Peck, Hannah Rotolo, Laura Zurla, Chiara
Source	BioRxiv
abstract	Here, Cas13a has been used to target and mitigate influenza virus A (IAV) and SARS-CoV-2 using a synthetic mRNA-based platform. CRISPR RNAs (crRNA) against PB1 and highly conserved regions of PB2 were screened in conjunction with mRNA-encoded Cas13a. Screens were designed such that only guides that decreased influenza RNA levels in a Cas13-mediated fashion, were valid. Cas13a mRNA and validated guides, delivered post-infection, simulating treatment, were tested in combination and across multiplicities of infection. Their function was also characterized over time. Similar screens were performed for guides against SARS-CoV-2, yielding multiple guides that significantly impacted cytopathic effect. Last, the approach was utilized in vivo, demonstrating the ability to degrade influenza RNA in a mouse model of infection, using polymer-formulated, nebulizer-based mRNA delivery. Our findings demonstrate the applicability of Cas13a in mitigating respiratory infections both in vitro and in a mouse model, paving the way for future therapeutic use.
has issue date	2020-04-24(xsd:dateTime)
bibo:doi	10.1101/2020.04.24.060418
has license	biorxiv
sha1sum (hex)	c83a92560df75650956a0344d6bf1382ef603fb8
schema:url	https://doi.org/10.1101/2020.04.24.060418
resource representing a document's title	Treating Influenza and SARS-CoV-2 via mRNA-encoded Cas13a
schema:publication	bioRxiv
resource representing a document's body	covid:c83a92560df75650956a0344d6bf1382ef603fb8#body_text
is schema:about of	named entity 'target' named entity 'levels' named entity 'SARS-CoV-2' named entity 'virus' named entity 'demonstrating' named entity 'influenza' named entity 'cytopathic' named entity 'platform' named entity 'synthetic' named entity 'conserved regions' named entity 'Treating' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'influenza' named entity 'mRNA' named entity 'infection' named entity 'infection' named entity 'nebulizer' named entity 'SARS-CoV-2' named entity 'mRNA' named entity 'Influenza' named entity 'influenza' named entity 'RNA' named entity 'mRNA' named entity 'mRNA' named entity 'bioinformatic' named entity 'substrate' named entity 'two-way analysis of variance' named entity 'protein' named entity 'mRNA' named entity 'polymer' named entity 'RNA' named entity 'GPI-anchored' named entity 'Plasmids' named entity 'influenza infection' named entity 'IAV' named entity 'animal group' named entity 'RNA' named entity 'gene expression' named entity 'influenza' named entity 'RNA viruses' named entity 'RNA' named entity 'conserved region' named entity 'RNA' named entity 'fluorescence' named entity 'lithium chloride' named entity 'PPIB' named entity 'GFP' named entity 'mRNAs' named entity 'nebulizer' named entity 'homology' named entity 'isoflurane' named entity 'reagent' named entity 'RNA' named entity 'drug development' named entity 'crRNA' named entity 'mRNA' named entity 'influenza' named entity 'Influenza' named entity 'mRNA' named entity 'lysed' named entity 'RNA' named entity 'biological replicates' named entity 'Ebola' named entity 'replicase' named entity 'mice' named entity 'mRNA' named entity 'Promega' named entity 'IAV' named entity 'anti-viral drugs'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software