About: Development of a Rhinovirus Inoculum Using a Reverse Genetics Approach

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a Rhinovirus Inoculum Using a Reverse Genetics Approach
Creator	Nakagome, Kazuyuki Lee, Iris Gern, James Togias, Alkis Grindle, Kristine Gangnon, Ronald Bochkov, Yury Crisafi, Gina Denlinger, Loren Evans, Michael Wolff, Michele Swenson, Cheri Lee, Wai Liggett, Stephen Sigelman, Steven
Source	PMC
abstract	BACKGROUND: Experimental inoculation is an important tool for common cold and asthma research. Producing rhinovirus (RV) inocula from nasal secretions has required prolonged observation of the virus donor to exclude extraneous pathogens. We produced a RV-A16 inoculum using reverse genetics and determined the dose necessary to cause moderate colds in seronegative volunteers. METHODS: The consensus sequence of RV-A16 from a previous inoculum was cloned, and inoculum virus was produced using reverse genetics techniques. After safety testing, volunteers were inoculated with either RV-A16 (n = 26) or placebo (n = 10), Jackson cold scores were recorded, and nasal secretions were tested for shedding of RV-A16 ribonucleic acid. RESULTS: The reverse genetics process produced infectious virus that was neutralized by specific antisera and had a mutation rate similar to conventional virus growth techniques. The 1000 median tissue culture infectious dose (TCID(50)) dose produced moderate colds in most individuals with effects similar to that of a previously tested conventional RV-A16 inoculum. CONCLUSIONS: Reverse genetics techniques produced a RV-A16 inoculum that can cause clinical colds in seronegative volunteers, and they also serve as a stable source of virus for laboratory use. The recombinant production procedures eliminate the need to derive seed virus from nasal secretions, thus precluding introduction of extraneous pathogens through this route.
has issue date	2019-07-15(xsd:dateTime)
bibo:doi	10.1093/infdis/jiy629
bibo:pmid	30383246
has license	no-cc
sha1sum (hex)	c6b2ffc5a86ad4dd61bdc1ff6aaea7bacf824679
schema:url	https://doi.org/10.1093/infdis/jiy629
resource representing a document's title	Development of a Rhinovirus Inoculum Using a Reverse Genetics Approach
has PubMed Central identifier	PMC6581892
has PubMed identifier	30383246
schema:publication	J Infect Dis
resource representing a document's body	covid:c6b2ffc5a86ad4dd61bdc1ff6aaea7bacf824679#body_text
is schema:about of	named entity 'seronegative' covid:arg/c6b2ffc5a86ad4dd61bdc1ff6aaea7bacf824679 named entity 'pathogens' named entity 'volunteers' named entity 'Rhinovirus' named entity 'common cold' named entity 'inoculation' named entity 'virus' named entity 'Inoculum' named entity 'inocula' named entity 'inoculation' named entity 'inoculation' named entity 'recombinant' named entity 'inoculation' named entity 'picornaviruses' named entity 'symptom' named entity 'colds' named entity 'inoculation' named entity 'colds' named entity 'Inoculation' named entity 'infectious agents' named entity 'de-escalation' named entity 'virus' named entity 'inoculum' named entity 'microbial' named entity 'viral genome' named entity 'rat' named entity 'viruses' named entity 'Secondary endpoints' named entity 'inoculum' named entity 'rotavirus vaccine' named entity 'cultured cells' named entity 'bovine' named entity 'Nasal lavage' named entity 'antiviral drugs' named entity 'inoculation' named entity 'plasmid' named entity 'chronic respiratory disease' named entity 'virus' named entity 'inoculation' named entity 'viruses' named entity 'tissue culture' named entity 'viruses' named entity 'alanine aminotransferase' named entity 'symptom' named entity 'inocula' named entity 'cloned' named entity 'young children' named entity 'deoxyribonucleic acid' named entity 'viruses' named entity 'inoculum' named entity 'inoculum' named entity 'cystic fibrosis' named entity 'epidemic' named entity 'platelets' named entity 'serum' named entity 'pathogens' named entity 'inoculum' named entity 'inocula' named entity 'inoculation' named entity 'RNA polymerases' named entity 'inoculum' named entity 'inoculation' named entity 'filtration' named entity 'GenBank' named entity 'Chicago' named entity 'community-acquired'

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