About: Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent

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About: Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent
Creator	Saunders, David Airs, Stephanie Eltringham, Gary Roberts, David Magee, John Sails, Andrew
Source	Elsevier; Medline; PMC
abstract	Two rapid real-time RT-PCR assays, specific for respiratory syncytial virus (RSV) and influenza A and B, were evaluated for the detection of these viruses in clinical respiratory samples. The RSV assay was applied to 100 samples and the Influenza A and B assay applied to 96 samples all of which had been tested previously by an “in-house” multiplex real-time PCR assay. Forty-three samples were negative for RSV by both methods and 56 samples were positive by both methods. One sample was negative by the new RSV assay although it was positive for RSV A by the “in-house” test. Thirty-nine samples were negative for influenza virus by both methods and 55 samples were positive by both assays. Two samples were negative by the new influenza assay however they were positive by the “in-house” influenza assay. The new assays did not cross react with samples containing other viruses including parainfluenza 1, 2, and 4; human metapnuemovirus; coronavirus 229E, NL63, OC43; rhinovirus; adenovirus; bocavirus and had a specificity of 100% and a sensitivity of 98.2% for RSV and 96.5% for influenza respectively. The results of this study demonstrate that the new assays are specific and sensitive for the detection of RSV and influenza viruses in clinical samples.
has issue date	2009-08-03(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.07.020
bibo:pmid	19651159
has license	no-cc
sha1sum (hex)	c623f5a2e88639ffa18ad6bdfa6545c52b791197
schema:url	https://doi.org/10.1016/j.jviromet.2009.07.020
resource representing a document's title	Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent
has PubMed Central identifier	PMC7119759
has PubMed identifier	19651159
schema:publication	J Virol Methods
resource representing a document's body	covid:c623f5a2e88639ffa18ad6bdfa6545c52b791197#body_text
is schema:about of	named entity 'influenza A' named entity 'assay' named entity 'specific' named entity 'samples' named entity 'Two' named entity 'virus' named entity 'real-time PCR' named entity 'assay' named entity 'negative' covid:arg/c623f5a2e88639ffa18ad6bdfa6545c52b791197 named entity 'influenza' named entity 'respiratory syncytial virus' named entity 'assays' named entity 'samples' named entity 'assays' named entity 'OC43' named entity 'RSV' named entity 'samples' named entity 'RSV' named entity 'A/B' named entity 'influenza' named entity 'NL63' named entity 'influenza virus' named entity 'rhinovirus' named entity 'coronavirus' named entity 'viruses' named entity 'real-time PCR' named entity 'RSV' named entity 'OC43' named entity 'clinical samples' named entity 'reagent' named entity 'real-time PCR' named entity 'Cepheid' named entity 'RSV' named entity 'influenza' named entity 'coronavirus' named entity 'treatment of patients' named entity 'Analyte' named entity 'respiratory disease' named entity 'viruses' named entity 'influenza' named entity 'concordance' named entity 'influenza' named entity 'multiplex assay' named entity 'parainfluenza virus' named entity 'cell culture' named entity 'Lewes' named entity 'real-time PCR instrument' named entity '229E' named entity 'random access' named entity 'influenza' named entity 'Qiagen' named entity 'influenza' named entity 'respiratory disease' named entity 'POCT' named entity 'RSV' named entity 'respiratory syncytial virus' named entity 'POCT' named entity 'RSV' named entity 'TaqMan' named entity 'influenza' named entity 'real-time RT-PCR' named entity 'real-time PCR' named entity 'RSV' named entity 'adenovirus' named entity 'adenovirus' named entity 'influenza' named entity 'coronavirus' named entity 'RSV' named entity 'false negative results' named entity 'cross-reactions' named entity 'nucleic acid' named entity 'adenovirus' named entity 'molecular diagnostic' named entity 'cell culture' named entity 'Influenza'

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