About: Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus

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About: Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus
Creator	Jung, Kwonil Annamalai, Thavamathi Eyerly, Bryan Lu, Zhongyan Saif, Linda
Source	Elsevier; Medline; PMC
abstract	Integrity of the intestinal epithelium is critical for proper functioning of the barrier that regulates absorption of water and restricts uptake of luminal bacteria. It is maintained mainly by tight junctions (TJs) and adherens junctions (AJs). We conducted immunofluorescence (IF) staining for in situ identification of zonula occludin (ZO)-1 proteins for TJ and E-Cadherin proteins for AJ in the small and large intestinal villous and crypt epithelium of nursing pigs infected with porcine epidemic diarrhea virus (PEDV). Twenty 9-day-old piglets [PEDV-infected (n = 9) and Mock (n = 11)] from PEDV seronegative sows, were orally inoculated [8.9 log(10) genomic equivalents/pig] with PEDV PC21A strain or mock. At post-inoculation days (PIDs) 1–5, infected pigs showed severe watery diarrhea and/or vomiting and severe atrophic enteritis. By immunohistochemistry, PEDV antigens were evident in enterocytes lining the villous epithelium. At PIDs 1–5, PEDV-infected pigs exhibited mildly to extensively disorganized, irregular distribution and reduced expression of ZO-1 or E-Cadherin in villous, but not crypt epithelial cells of the jejunum and ileum, but not in the large intestine, when compared to the negative controls. The structural destruction and disorganization of TJ and AJ were extensive in PEDV-infected pigs at PIDs 1–3, but then appeared to reversibly recover at PID 5, as evident by increased numbers of ZO-1-positive epithelial cells and markedly improved appearance of E-Cadherin-positive villous epithelium. Our results suggest a possible involvement of structurally impaired TJ and AJ in the pathogenesis of PEDV, potentially leading to secondary bacterial infections.
has issue date	2015-06-12(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2015.03.022
bibo:pmid	25843943
has license	no-cc
sha1sum (hex)	c5eafac56a54ae00c02fd71344e6f5642d41ee3a
schema:url	https://doi.org/10.1016/j.vetmic.2015.03.022
resource representing a document's title	Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus
has PubMed Central identifier	PMC7117115
has PubMed identifier	25843943
schema:publication	Vet Microbiol
resource representing a document's body	covid:c5eafac56a54ae00c02fd71344e6f5642d41ee3a#body_text
is schema:about of	named entity 'numbers' named entity 'secondary' named entity 'luminal' named entity 'recover' named entity 'zonula' named entity 'barrier' named entity 'water' named entity 'villous' named entity 'Structural' named entity 'crypt' named entity 'IN SITU' named entity 'POSSIBLE' named entity 'POSITIVE' named entity 'SUGGEST' named entity 'E-CADHERIN' named entity 'TIGHT JUNCTIONS' named entity 'INTESTINAL EPITHELIUM' named entity 'INVOLVEMENT' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'MOCK' named entity 'POST' named entity 'IMMUNOFLUORESCENCE' named entity 'IDENTIFICATION' named entity 'STRUCTURAL' named entity 'EXTENSIVE' named entity 'LEADING' named entity 'ZONULA' named entity 'IMPAIRED' named entity 'INTEGRITY OF' named entity 'LINING' named entity 'ENTERITIS' named entity 'SOWS' named entity 'RECOVER' named entity 'SMALL' named entity 'SECONDARY' named entity 'ABSORPTION' named entity 'CRITICAL' named entity 'STAINING' named entity 'INFECTED' named entity 'ATROPHIC' named entity 'IMPROVED' named entity 'BUT' named entity 'RESTRICTS' named entity 'TWENTY' named entity 'SERONEGATIVE' named entity 'STRAIN' named entity 'LOG' named entity 'MAINTAINED' named entity 'NUMBERS' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PROPER' named entity 'PID' named entity 'REDUCED' named entity 'BACTERIAL INFECTIONS' named entity 'BY INCREASED' named entity 'EPITHELIAL CELLS' named entity 'PROTEINS' named entity 'DAYS' named entity 'NEGATIVE' named entity 'ADHERENS JUNCTIONS' named entity 'CRYPT EPITHELIUM' named entity 'ADHERENS JUNCTIONS' named entity 'LARGE INTESTINE' named entity 'VILLOUS' named entity 'OCCLUDIN' named entity 'SEVERE' named entity 'PATHOGENESIS' named entity 'VILLOUS' named entity 'RESULTS' named entity 'TIGHT' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'INFECTED' named entity 'NURSING' named entity 'ILEUM' named entity 'ORALLY' named entity 'CRYPT EPITHELIUM' named entity 'VILLOUS EPITHELIUM'

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