About: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus
Creator	Chen, Qi Zhang, Yan Gauger, Phillip Harmon, Karen Zhang, Jianqiang Tsai, Yun-Long Lee, Pei-Yu Chang, Hsiao-Fen Chiang, Cheng-Jen Li, Fu-Chun Shen, Yu-Han Wang, Thomas
Source	Elsevier; Medline; PMC
abstract	Abstract Recent outbreaks of porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) in multiple countries have caused significant economic losses and remain a serious challenge to the swine industry. Rapid diagnosis is critical for the implementation of efficient control strategies before and during PEDV and PDCoV outbreaks. Insulated isothermal PCR (iiPCR) on the portable POCKIT™ device is user friendly for on-site pathogen detection. In the present study, a singleplex PEDV RT-iiPCR, a singleplex PDCoV RT-iiPCR, and a duplex PEDV/PDCoV real-time RT-PCR (rRT-PCR) commercial reagents targeting the M gene were compared to an N gene-based PEDV rRT-PCR and an M gene-based PDCoV rRT-PCR that were previously published and used as reference PCRs. All PCR assays were highly specific and did not cross react with other porcine enteric pathogens. Analytical sensitivities of the PEDV RT-iiPCR, PDCoV RT-iiPCR and duplex PEDV/PDCoV rRT-PCR were determined using in vitro transcribed RNA as well as viral RNA extracted from ten-fold serial dilutions of PEDV and PDCoV cell culture isolates. Performance of each PCR assay was further evaluated using 170 clinical samples (86 fecal swabs, 24 feces, 19 intestines, and 41 oral fluids). Compared to the reference PEDV rRT-PCR, the sensitivity, specificity and accuracy of the PEDV RT-iiPCR were 97.73%, 98.78%, and 98.24%, respectively, and those of the duplex PEDV/PDCoV rRT-PCR were 98.86%, 96.34%, and 97.65%, respectively. Compared to the reference PDCoV rRT-PCR, the sensitivity, specificity and accuracy of the PDCoV RT-iiPCR were 100%, 100%, and 100%, respectively, and those of the PEDV/PDCoV duplex rRT-PCR were 96.34%, 100%, and 98.24%, respectively. Overall, all three new PCR assays were comparable to the reference rRT-PCRs for detection of PEDV and/or PDCoV. The PEDV and PDCoV RT-iiPCRs are potentially useful tools for on-site detection and the duplex PEDV/PDCoV rRT-PCR provides a convenient method to simultaneously detect the two viruses and differentiate PEDV from PDCoV.
has issue date	2016-08-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2016.03.016
bibo:pmid	27060624
has license	els-covid
sha1sum (hex)	c58715798b7ae050186df99882c4f62fd73c2916
schema:url	https://doi.org/10.1016/j.jviromet.2016.03.016
resource representing a document's title	Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus
has PubMed Central identifier	PMC7113669
has PubMed identifier	27060624
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:c58715798b7ae050186df99882c4f62fd73c2916#body_text
is schema:about of	named entity 'compared' named entity 'diagnosis' named entity 'DETECTION' covid:arg/c58715798b7ae050186df99882c4f62fd73c2916 named entity 'DUPLEX' named entity 'M GENE' named entity 'USER' named entity 'NEW' named entity 'TEST' named entity 'SAMPLES' named entity 'RAPID DIAGNOSIS' named entity 'METHOD' named entity 'PRESENT' named entity 'REAL-TIME RT-PCR' named entity 'FECES' named entity 'IMPLEMENTATION' named entity 'IN VITRO' named entity 'DEVICE' named entity 'SPECIFICITY' named entity 'TARGETING' named entity 'CROSS' named entity 'EXTRACTED' named entity 'EFFICIENT' named entity 'SIMULTANEOUSLY' named entity 'REAGENTS' named entity 'CONVENIENT' named entity 'VIRUSES' named entity 'STRATEGIES' named entity 'OF EACH' named entity 'PATHOGEN DETECTION' named entity 'TOOLS' named entity 'DID' named entity 'FLUIDS' named entity 'COUNTRIES' named entity 'COMPARED' named entity 'PCR ASSAY' named entity 'ACCURACY' named entity 'OVERALL' named entity 'HAVE' named entity 'PCR' named entity 'CAUSED' named entity 'USING' named entity 'PROVIDES' named entity 'PORCINE DELTACORONAVIRUS' named entity 'REAL-TIME RT-PCR' named entity 'FRIENDLY' named entity 'PORTABLE' named entity 'EVALUATED' named entity 'CHALLENGE' named entity 'ISOLATES' named entity 'FECAL' named entity 'TESTS' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PCR' named entity 'DETECTION' named entity 'ANALYTICAL' named entity 'DUPLEX' named entity 'CELL CULTURE' named entity 'ORAL' named entity 'ENTERIC' named entity 'HIGHLY' named entity 'OUTBREAKS' named entity 'SITE' named entity 'REVERSE TRANSCRIPTION' named entity 'EVALUATION' named entity 'PORCINE DELTACORONAVIRUS' named entity 'INTESTINES' named entity '170' named entity 'PERFORMANCE' named entity 'CONTROL'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software