About: H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes

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About: H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes
Creator	Amonsin, Alongkorn Poovorawan, Yong Theamboonlers, Apiradee Damrongwatanapokin, Sudarat Chieochansin, Thaweesak Suwannakarn, Kamol Landt, Olfert Chaisingh, Arunee Chutinimitkul, Salin Mai, Le Songserm, Thaweesak
source	Elsevier; Medline; PMC
abstract	A single amino acid substitution, from histidine to tyrosine at position 274 of the neuraminidase gene has converted Oseltamivir sensitive H5N1 influenza A virus into a resistant strain. Currently, Oseltamivir is being stockpiled in many countries potentially affected by the influenza A virus subtype H5N1 epidemic. To identify this change in Oseltamivir-treated patients, a method based on real-time PCR using two labeled TaqMan probes was developed for its rapid detection. In order to validate the method, Oseltamivir specimen from treated (Oseltamivir-resistant strain from a Vietnamese patient, two Oseltamivir-treated tigers) and untreated subjects have been used for this study. The results thus obtained as well as those derived from clone selection and sequencing showed that TaqMan probes could clearly discriminate wild type H274 from the mutant 274Y variant. The sensitivity of this assay was as low as 10 copies/μl and allowed the detection of the mutation in a mixture of wild type and mutant. Overall, the assay based on real-time PCR with two labeled TaqMan probes described here should be useful for detecting Oseltamivir-resistant H274Y H5N1 influenza A virus in many species and various sources of specimens with high sensitivity and specificity. Such studies can address potential differences in the diagnostic outcomes between patients who develop detectable Oseltamivir resistance and those who retain only the wild type strain of H5N1.
has issue date	2006-10-19(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.09.007
bibo:pmid	17055070
has license	no-cc
sha1sum (hex)	c5169d846b482e36f04c6d2224a56d9cce256087
schema:url	https://doi.org/10.1016/j.jviromet.2006.09.007
resource representing a document's title	H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes
has PubMed Central identifier	PMC7112789
has PubMed identifier	17055070
schema:publication	J Virol Methods
resource representing a document's body	covid:c5169d846b482e36f04c6d2224a56d9cce256087#body_text
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