About: Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes

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About: Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes
Creator	Bergallo, Massimiliano Astegiano, Sara Cavallo, Rossana Sidoti, Francesca Gasparini, Giorgio Maria, • Piasentin, Elsa Terlizzi, Elena
Source	PMC
abstract	Evidence demonstrating that human rhinovirus (HRV) disease is not exclusively limited to the upper airways and may cause lower respiratory complications, together with the frequency of HRV infections and the increasing number of immunocompromised patients underline the need for rapid and accurate diagnosis of HRV infections. In this study, we developed the first quantitative real-time nucleic acid sequence-based amplification assay with an internal control using molecular beacon probes for selective and sensitive detection of human rhinovirus serotypes. We described a simple method to accurately quantify RNA target by computing the time to positivity (TTP) values for HRV RNA. Quantification capacity was assessed by plotting these TTP values against the starting number of target molecules. By using this simple method, we have significantly increased the diagnostic accuracy, precision, and trueness of real-time NASBA assay. Specificity of the method was verified in both in silico and experimental studies. Moreover, for assessment of clinical reactivity of the assay, NASBA has been validated on bronchoalveolar lavage (BAL) specimens. Our quantitative NASBA assay was found to be very specific, accurate, and precise with high repeatability and reproducibility.
has issue date	2011-07-05(xsd:dateTime)
bibo:doi	10.1007/s12033-011-9432-4
bibo:pmid	21728031
has license	no-cc
sha1sum (hex)	c515bc2f154c41d35b7c4fac6686284f9091a49e
schema:url	https://doi.org/10.1007/s12033-011-9432-4
resource representing a document's title	Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes
has PubMed Central identifier	PMC7091405
has PubMed identifier	21728031
schema:publication	Mol Biotechnol
resource representing a document's body	covid:c515bc2f154c41d35b7c4fac6686284f9091a49e#body_text
is schema:about of	named entity 'QUANTITATIVE' named entity 'accurate' named entity 'specimens' named entity 'rapid' named entity 'sensitive' named entity 'plotting' named entity 'DETECTION' named entity 'CONTROL' named entity 'MOLECULAR BEACON' named entity 'REPRODUCIBILITY' named entity 'STUDY' named entity 'TTP' named entity 'COMPUTING' named entity 'PRECISE' named entity 'TIME' named entity 'ASSESSED' named entity 'SENSITIVE' named entity 'BRONCHOALVEOLAR LAVAGE' named entity 'VALIDATED' named entity 'VERIFIED' named entity 'INFECTIONS' named entity 'CLINICAL' named entity 'SIMPLE' named entity 'USING' named entity 'AMPLIFICATION' named entity 'IMMUNOCOMPROMISED PATIENTS' named entity 'TARGET' named entity 'EXPERIMENTAL STUDIES' named entity 'SEROTYPES' named entity 'DETECTION' named entity 'INCREASING' named entity 'THESE' named entity 'QUANTIFY' named entity 'RESPIRATORY COMPLICATIONS' named entity 'REAL-TIME' named entity 'USING' named entity 'SEROTYPES' named entity 'DEVELOPMENT' named entity 'HRV' named entity 'NUCLEIC ACID SEQUENCE' named entity 'LIMITED' named entity 'ASSAY' named entity 'SELECTIVE' named entity 'ACCURATE' named entity 'PROBES' named entity 'NEED FOR' named entity 'SPECIFIC' named entity 'DIAGNOSTIC ACCURACY' named entity 'DESCRIBED' named entity 'FREQUENCY' named entity 'MOLECULES' named entity 'DISEASE' named entity 'NUCLEIC ACID SEQUENCE' named entity 'EVIDENCE' named entity 'REAL-TIME' named entity 'MOLECULAR BEACON' named entity 'HUMAN RHINOVIRUS' named entity 'IN SILICO' named entity 'INTERNAL' named entity 'ASSAY' named entity 'PROBES' named entity 'DIAGNOSIS' named entity 'CAUSE' named entity 'NUMBER OF' named entity 'VALUES' named entity 'RAPID' named entity 'QUANTIFICATION' named entity 'REPEATABILITY' named entity 'AIRWAYS' named entity 'CONTROL' named entity 'INCREASED' named entity 'LOWER' named entity 'PRECISION' named entity 'HAVE'

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