About: Far‐Red Fluorescent Lipid‐Polymer Probes for an Efficient Labeling of Enveloped Viruses

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About: Far‐Red Fluorescent Lipid‐Polymer Probes for an Efficient Labeling of Enveloped Viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Far‐Red Fluorescent Lipid‐Polymer Probes for an Efficient Labeling of Enveloped Viruses
Creator	Pécheur, Eve-Isabelle Blaising, Julie Adjili, Salim Blaising, J Charreyre, Marie-Thérèse Favier, Arnaud Lacour, William Ladavière, Catherine Mezhoud, Sarra Monier, Karine Place, C Place, Christophe Pécheur, E.-I
Source	PMC
abstract	Far‐red emitting fluorescent lipid probes are desirable to label enveloped viruses, for their efficient tracking by optical microscopy inside autofluorescent cells. Most used probes are rapidly released from membranes, leading to fluorescence signal decay and loss of contrast. Here, water‐soluble lipid‐polymer probes are synthesized harboring hydrophilic or hydrophobic far‐red emitting dyes, and exhibiting enhanced brightness. They efficiently label Hepatitis C Virus pseudotyped particles (HCVpp), more stably and reproducibly than commercial probes, and a strong fluorescence signal is observed with a high contrast. Labeling with such probes do not alter virion morphology, integrity, nor infectivity. Finally, it is shown by fluorescence microscopy that these probes enable efficient tracking of labeled HCVpp inside hepatocarcinoma cells used as model hepatocytes, in spite of their autofluorescence up to 700 nm. These novel fluorescent lipid‐polymer probes should therefore enable a better characterization of early stages of infection of autofluorescent cells by enveloped viruses.
has issue date	2016-04-26(xsd:dateTime)
bibo:doi	10.1002/adhm.201600091
bibo:pmid	27113918
has license	no-cc
sha1sum (hex)	c3ed28c18e3cee4f064a82339cec5f1dd1a35103
schema:url	https://doi.org/10.1002/adhm.201600091
resource representing a document's title	Far‐Red Fluorescent Lipid‐Polymer Probes for an Efficient Labeling of Enveloped Viruses
has PubMed Central identifier	PMC7159338
has PubMed identifier	27113918
schema:publication	Adv Healthc Mater
resource representing a document's body	covid:c3ed28c18e3cee4f064a82339cec5f1dd1a35103#body_text
is schema:about of	named entity 'strong' named entity 'morphology' named entity 'virion' named entity 'probes' named entity 'enhanced' named entity 'RED' named entity 'ENHANCED' named entity 'MEMBRANES' named entity 'DO NOT' named entity 'VIRUSES' named entity 'enable' named entity 'emitting' named entity 'Here' named entity 'model' named entity 'probes' named entity 'loss' named entity 'cells' named entity 'hydrophilic' named entity 'far-red' named entity 'pseudotyped' named entity 'morphology' named entity 'lipid' named entity 'infection' named entity 'hepatocarcinoma' named entity 'water-soluble' named entity 'Enveloped Viruses' named entity 'tracking' named entity 'signal' named entity 'polyclonal antibody' named entity 'embryos' named entity 'intracellular' named entity 'antibodies' named entity 'Molecular Devices' named entity 'electrostatic repulsions' named entity 'transmission electron microscopy' named entity 'TX2' named entity 'far-red' named entity 'biotin' named entity 'Huh7' named entity 'NaCl' named entity 'Invitrogen' named entity 'dialysis' named entity 'activated ester' named entity '100 nm' named entity 'HCV' named entity 'monomer units' named entity 'Gag-Pol' named entity 'polymer' named entity 'streptomycin' named entity '0.45' named entity 'DPPC' named entity 'Erythrosin' named entity 'inside the cell' named entity 'dye' named entity 'polymer' named entity 'membrane systems' named entity 'deprotonated' named entity 'DIPEA' named entity 'HPLC' named entity '30 nm' named entity 'Supernatants' named entity 'cytotoxicity' named entity 'lipid envelope' named entity 'far-red' named entity 'microscopy' named entity 'therapeutic targets' named entity 'genotype' named entity 'optical microscopy' named entity 'hepatocytes'

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