About: Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D

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About: Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D
Creator	Wang, Ping Dai, Yunping Wang, Meili Zheng, Min Chen, X Bowen, Yan Cheng, Xueqian Huang, Tan Kemian, Gou Wang, Xifeng Yi, Sun Zhiguo, Wei
Source	Medline; PMC
abstract	Mutation of mevalonate kinase (MVK) is thought to account for most cases of hyperimmunoglobulinemia D syndrome (HIDS) with recurrent fever. However, its mechanism and the relationship between elevated serum immunoglobulin D (IgD) and the clinical features of HIDS are unclear. In this study, we generated by fusion PCR a vector to express high levels of chimeric secretory IgD (csIgD) specifically in the liver. We then generated seven founder lines of transgenic mice by co-microinjection, and verified them using genomic PCR and Southern blotting. We detected the expression of csIgD by reverse transcription PCR, quantitative PCR, western blotting, and enzyme-linked immunosorbent assays. We demonstrated that csIgD could be specifically and stably expressed in the liver. We used flow cytometry to show that overexpression of csIgD in the bone marrow and spleen cells had no effect on B cell development. Morphologic and anatomical observation of the transgenic mice revealed skin damage, hepatosplenomegaly, and nephromegaly in some transgenic mice; in these mice, pathological sections showed high levels of cell necrosis and protein-like sediments in the liver, spleen, and kidney. We demonstrated that the genomic insertion sites of the transgenes did not disrupt the MVK gene on mouse chromosome 5. This transgenic mouse will be useful to explore the pathogenesis of HIDS.
has issue date	2012-04-14(xsd:dateTime)
bibo:doi	10.1007/s11427-012-4301-3
bibo:pmid	22527518
has license	cc-by
sha1sum (hex)	c3cca3d66fd6ef068573a246a76360a9e574cad1
schema:url	https://doi.org/10.1007/s11427-012-4301-3
resource representing a document's title	Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D
has PubMed Central identifier	PMC7089205
has PubMed identifier	22527518
schema:publication	Sci China Life Sci
resource representing a document's body	covid:c3cca3d66fd6ef068573a246a76360a9e574cad1#body_text
is schema:about of	named entity 'MVK' named entity 'MVK' named entity 'generated' named entity 'overexpression' named entity 'mechanism' named entity 'liver' named entity 'sediments' named entity 'necrosis' named entity 'IgD' named entity 'generated' named entity 'enzyme-linked immunosorbent assays' named entity 'LINES' named entity 'DETECTED' named entity 'INSERTION' named entity 'MVK GENE' named entity 'CASES' named entity 'TRANSGENES' named entity 'ELEVATED' named entity 'SKIN DAMAGE' named entity 'DEMONSTRATED' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'FUSION' named entity 'CELLS' named entity 'VECTOR' named entity 'COULD BE' named entity 'LIKE' named entity 'TO EXPRESS' named entity 'IMMUNOGLOBULIN D' named entity 'CLINICAL FEATURES' named entity 'THESE' named entity 'MICE' named entity 'MICROINJECTION' named entity 'ANATOMICAL' named entity 'PATHOLOGICAL' named entity 'REVEALED' named entity 'HYPERIMMUNOGLOBULINEMIA D SYNDROME' named entity 'B CELL DEVELOPMENT' named entity 'RELATIONSHIP' named entity 'MEVALONATE KINASE' named entity 'SEVEN' named entity 'PCR' named entity 'USED' named entity 'KIDNEY' named entity 'MUTATION' named entity 'SOUTHERN BLOTTING' named entity 'PROTEIN' named entity 'EXPRESSED' named entity 'UNCLEAR' named entity 'SERUM IMMUNOGLOBULIN' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAYS' named entity 'OVEREXPRESSION' named entity 'FOUNDER' named entity 'DID' named entity 'USEFUL' named entity 'VERIFIED' named entity 'LIVER' named entity 'EXPRESSION' named entity 'HIDS' named entity 'MECHANISM' named entity 'SECRETORY' named entity 'GENERATED' named entity 'PATHOGENESIS' named entity 'QUANTITATIVE PCR' named entity 'TRANSGENIC MOUSE' named entity 'MOUSE MODEL' named entity 'IMMUNOGLOBULIN D' named entity 'ESTABLISHMENT' named entity 'USING' named entity 'EXPRESSION' named entity 'CHINA'

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