About: Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV)

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About: Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV)
Creator	Lavazza, Antonio Lelli, Davide Moreno, Ana Prosperi, Alice Sozzi, Enrica Papetti, Alice Capucci, Lorenzo Alborali, Giovanni Giacomini, Enrico Perulli, Simona Brocchi, Emiliana
source	Elsevier; Medline; PMC
abstract	Abstract Porcine epidemic diarrhoea virus (PEDV), belongs to the genus Alphacoronavirus in the family Coronaviridae and causes severe diarrhoea, vomiting, dehydration and high mortality in seronegative newborn piglets. Thus, a precise and rapid diagnosis of PEDV infection is important for the application of control measures to limit viral dissemination. In this investigation, a monoclonal antibodies (MAbs)-based competitive enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against PEDV was developed and validated. The diagnostic performance of the test was evaluated by receiver operating characteristic (ROC) analysis using a panel of 829 known sera collected from different commercial pig farms, with or without a history of PED presence and from experimentally infected pigs. The competitive ELISA showed excellent diagnostic performance and discriminatory power with high sensitivity (Se) and specificity (Sp) values (Se = 96.5%, 95% IC 94.1–98.1; Sp = 98.2%, 95% IC 96.3–99.3). Moreover, this competitive ELISA method has other properties, such as high feasibility of testing sera without pre-treatment and automatic and instrument-mediated revealing, that makes it appropriate for large-scale screenings of affected pig farms in endemic regions or for monitoring plans in PEDV-free areas.
has issue date	2018-12-31(xsd:dateTime)
bibo:doi	10.1016/j.rvsc.2018.10.011
bibo:pmid	30384140
has license	els-covid
sha1sum (hex)	c1dd2ac57ae412f88c85d3fd4afce636b168e6fe
schema:url	https://doi.org/10.1016/j.rvsc.2018.10.011
resource representing a document's title	Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV)
has PubMed Central identifier	PMC7111896
has PubMed identifier	30384140
schema:publication	Research in Veterinary Science
resource representing a document's body	covid:c1dd2ac57ae412f88c85d3fd4afce636b168e6fe#body_text
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