About: Cloning whole bacterial genomes in yeast

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cloning whole bacterial genomes in yeast
Creator	Alperovich, Nina Assad-Garcia, Nacyra Carrera, William Chuang, Ray-Yuan Lartigue, Carole Merryman, Chuck Moodie, Monzia Phan, Quang Vashee, Sanjay Algire, Mikkel Benders, Gwynedd Craig Venter, J Denisova, Evgeniya Gibson, Daniel Glass, John Hutchison Iii, Clyde Noskov, Vladimir Smith, Hamilton
Source	Medline; PMC
abstract	Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.
has issue date	2010-03-07(xsd:dateTime)
bibo:doi	10.1093/nar/gkq119
bibo:pmid	20211840
has license	cc-by-nc
sha1sum (hex)	c147fd685f974994cea6cb2329084754f655062c
schema:url	https://doi.org/10.1093/nar/gkq119
resource representing a document's title	Cloning whole bacterial genomes in yeast
has PubMed Central identifier	PMC2860123
has PubMed identifier	20211840
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:c147fd685f974994cea6cb2329084754f655062c#body_text
is schema:about of	named entity 'Most' named entity 'BACTERIAL GENOMES' covid:arg/c147fd685f974994cea6cb2329084754f655062c named entity 'THESE' named entity 'HERE' named entity 'GENOME' named entity 'PLASMIDS' named entity 'GENETIC' named entity 'SACCHAROMYCES CEREVISIAE' named entity 'CIRCULAR' named entity 'METHODS' named entity 'INTRACTABLE' named entity 'FRACTIONS' named entity 'CLONING' named entity 'YEAST' named entity 'SUBSPECIES' named entity 'DISCOVERY' named entity 'ESTABLISHED' named entity 'REPORT' named entity 'THEIR' named entity 'DNA MOLECULES' named entity 'ROUTINE' named entity 'LARGE' named entity 'HOST' named entity 'DIFFICULT' named entity 'CLONED' named entity 'WHOLE GENOMES' named entity 'MAINTAINED' named entity 'DNA' named entity 'CULTURED' named entity 'CLONING' named entity 'EFFICIENT' named entity 'CAPRI' named entity 'PNEUMONIAE' named entity 'APPEAR' named entity 'BACTERIAL GENOMES' named entity 'MYCOPLASMA GENITALIUM' named entity 'ENHANCE' named entity 'YEAST' named entity 'MANIPULATION' named entity 'SINGLE' named entity 'HAVE' named entity 'MICROBES' named entity 'ORGANISMS' named entity 'CHARACTERIZATION' named entity 'FUNCTIONAL' named entity 'M. mycoides' named entity 'cloning' named entity 'Routine' named entity 'cloned' named entity 'genomes' named entity 'genome' named entity 'enhance' named entity 'centromeric' named entity 'plasmids' named entity 'bacterial genomes' named entity 'yeast' named entity 'transposase' named entity 'PCR' named entity 'M. mycoides' named entity 'M. capricolum' named entity 'B. subtilis' named entity 'vector' named entity 'gene'

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