About: Localization of rhinovirus replication in vitro with in situ hybridization

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Localization of rhinovirus replication in vitro with in situ hybridization
Creator	Hayden, Frederick Winther, Birgit Gwaltney, Jack De Arruda, Eurico Mifflin, Theodore
Source	PMC
abstract	To facilitate understanding of human rhinovirus (HRV) pathogenesis, methods were developed for detection of HRV infection in vitro using in situ hybridization (ISH). HRV‐14 RNA probes and oligonucleotide probes representing conserved sequences in the 5′‐non‐translated region were labeled with (35)S and used to detect infected HeLa or WI‐38 strain human embryonic lung cells in cytological preparations. ISH was shown to be specific for detection of HRV on a single‐cell basis. Subsequently, in human nasal polyps infected in vitro, both oligonucleotide‐ and riboprobes produced a strong signal in association with ciliated epithelial cells. In human adenoids infected in vitro, a signal was observed in nonciliated epithelial cells. This study shows that HRV replicates in ciliated cells in the epithelium of human nasal polyps infected in vitro, and the presence of viral RNA in non‐ciliated cells of the human adenoid infected in vitro suggests that other cell types may also support rhinovirus replication.
has issue date	2005-12-07(xsd:dateTime)
bibo:doi	10.1002/jmv.1890340107
bibo:pmid	1653307
has license	no-cc
sha1sum (hex)	c043324f04796ac5c9bc44b52b9747c1421040c0
schema:url	https://doi.org/10.1002/jmv.1890340107
resource representing a document's title	Localization of rhinovirus replication in vitro with in situ hybridization
has PubMed Central identifier	PMC7166441
has PubMed identifier	1653307
schema:publication	J Med Virol
resource representing a document's body	covid:c043324f04796ac5c9bc44b52b9747c1421040c0#body_text
is schema:about of	named entity 'situ' named entity 'RNA' named entity 'epithelial cells' named entity 'detection' named entity 'icat' named entity 'cytological' named entity 'SUPPORT' named entity 'OLIGONUCLEOTIDE PROBES' named entity '35S' named entity 'SPECIFIC' named entity 'STUDY' named entity 'HELA' named entity 'USED' named entity 'HRV-14' named entity 'ION' named entity 'INFECTED' named entity 'EMBRYONIC' named entity 'REGION' named entity 'LUNG' named entity 'ASSOCIATION' named entity 'IN SITU HYBRIDIZATION' named entity 'SEQUENCES' named entity 'pathogenesis' named entity 'HeLa' named entity 'ISH' named entity 'oligonucleotides' named entity 'adenoid' named entity 'RNase' named entity 'lymphoid follicles' named entity 'genome sequences' named entity 'cell type' named entity 'genome' named entity 'sperm' named entity 'epithelial cells' named entity 'respiratory tract' named entity 'recombinant' named entity 'polyps' named entity 'influenza A virus' named entity 'dehydrated' named entity 'confounder' named entity 'lamina propria' named entity 'RNase' named entity 'Merck Sharp and Dohme Research Laboratories' named entity 'infection' named entity 'WI-38' named entity 'virus' named entity 'HRV' named entity 'HRV' named entity 'plasmid' named entity 'coverslip' named entity 'WI-38' named entity 'HRV' named entity 'dehydrated' named entity 'epithelial cells' named entity 'ciliated cells' named entity 'Walkersville' named entity 'ciliated' named entity 'triethanolamine' named entity 'humidified' named entity 'signal' named entity 'PBS' named entity 'RNase' named entity 'cell membrane' named entity 'rhinovirus' named entity 'assay' named entity 'inoculated' named entity 'epithelial cells' named entity 'ciliated cells' named entity 'nasal secretions' named entity 'HRV' named entity 'nasal mucosa' named entity 'serum' named entity 'rhinovirus' named entity 'dehydrated' named entity 'oligonucleotide probes' named entity 'silica gel' named entity 'cytocentrifuge'

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