About: Regulation of the Initiation of Coronavirus JHM Infection in Primary Oligodendrocytes and L-2 Fibroblasts

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About: Regulation of the Initiation of Coronavirus JHM Infection in Primary Oligodendrocytes and L-2 Fibroblasts Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Regulation of the Initiation of Coronavirus JHM Infection in Primary Oligodendrocytes and L-2 Fibroblasts
Creator	Dales, Samuel Kalicharran, Kishna Mohandas, Devaki Wilson, Greame
Source	Elsevier; Medline; PMC
abstract	Abstract Upon maturation, primary rat oligodendrocytes become resistant to coronavirus JHM (JHMV) infection at an early stage. Involvement of cAMP-dependent protein kinase (PK) in the regulation of oligodendrocyte differentiation has been established (S. Beushausenet al.(1987).J. Virol.61, 3795–3803). An inducer which accelerates maturation, dibutyryl cyclic AMP (dbcAMP) also upregulates the expression of the regulatory subunit, R1 of PK1. Since (i) early block preventing infection of mature oligodendrocytes can be bypassed when transfection with genomic RNA is used and (ii) inhibitors of PKs counteract the dbcAMP effect, so as to alleviate the inhibition of JHMV, enhanced expression of R1 appeared to be connected with virus restriction. This idea was confirmed following upregulation of the R1 gene in fully permissive L-2 cells. There was a connection between an effect due to R1 and dephosphorylation of the nucleocapsid protein N by an endosomal phosphoprotein phosphatase (PPPase) having the properties of types 1 or 2A enzyme which occurs during penetration of inoculum virions. An inhibitionin vitro(cell free) of N dephosphorylation by R1 together with evidence thatin vivo(cell culture) overexpression of R1 inhibited the endosomal PPPase as well as replication of JHMV supports the hypothesis that uncoating of the JHMV inoculum occurs after dephosphorylation, a step obligatory for dissociation of the N protein from the genome. Thus inhibition by R prevents uncoating and thereby interferes with the commencement of replication. These observations intimate the existence of a novel mechanism controlling a virus infection of specific cell target(s) undergoing a process of differentiation and maturation in the central nervous system.
has issue date	1996-11-01(xsd:dateTime)
bibo:doi	10.1006/viro.1996.0572
bibo:pmid	8918531
has license	els-covid
sha1sum (hex)	bfc558a4241a48e116131d26ece914f715b53d10
schema:url	https://doi.org/10.1006/viro.1996.0572
resource representing a document's title	Regulation of the Initiation of Coronavirus JHM Infection in Primary Oligodendrocytes and L-2 Fibroblasts
has PubMed Central identifier	PMC7131831
has PubMed identifier	8918531
schema:publication	Virology
resource representing a document's body	covid:bfc558a4241a48e116131d26ece914f715b53d10#body_text
is schema:about of	named entity 'infection' named entity 'differentiation' named entity 'overexpression' named entity 'mechanism' named entity 'stage' named entity 'Regulation' named entity 'REGULATION' named entity 'OLIGODENDROCYTES' named entity 'GENOME' named entity 'CONTROLLING' named entity 'INDUCER' named entity 'EARLY STAGE' named entity 'IN VITRO' named entity 'PK1' named entity 'INHIBITED' named entity 'VIRIONS' named entity 'PREVENTING' named entity 'NUCLEOCAPSID PROTEIN' named entity 'EXPRESSION' named entity 'OCCURS DURING' named entity 'VIRUS INFECTION' named entity 'RESTRICTION' named entity 'DEPHOSPHORYLATION' named entity 'PROPERTIES' named entity 'EARLY' covid:arg/bfc558a4241a48e116131d26ece914f715b53d10 named entity 'USED' named entity 'OVEREXPRESSION' named entity 'CAMP-DEPENDENT PROTEIN KINASE' named entity 'TYPES' named entity 'WAS A' named entity 'SUBUNIT' named entity 'PHOSPHOPROTEIN PHOSPHATASE' named entity 'FULLY' named entity 'FREE' named entity 'INVOLVEMENT' named entity 'OLIGODENDROCYTES' named entity 'CULTURE' named entity 'TRANSFECTION' named entity 'VIROL' named entity 'L-2' named entity 'PROTEIN ' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'INFECTION' named entity 'L-2' named entity 'INITIATION' named entity 'PRIMARY' named entity 'CELLS' named entity 'NOVEL' named entity 'EFFECT' named entity 'RNA' named entity 'CORONAVIRUS' named entity 'THESE' named entity 'OBSERVATIONS' named entity 'FOLLOWING' named entity 'INTIMATE' named entity '28I' named entity 'FIBROBLASTS' named entity 'PROCESS' named entity 'N PROTEIN' named entity 'PENETRATION' named entity '28S' named entity 'IDEA' named entity 'REGULATION OF OLIGODENDROCYTE DIFFERENTIATION' named entity 'PKS' named entity 'HYPOTHESIS' named entity 'SPECIFIC' named entity 'MATURE' named entity 'R1 GENE' named entity 'INHIBITORS' named entity 'ENZYME' named entity 'DIFFERENTIATION' named entity 'CONNECTION' named entity 'GENOMIC' named entity 'INHIBITION' named entity 'ENHANCED' named entity 'COMMENCEMENT' named entity 'ACCELERATES'

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