About: Site specific target binding controls RNA cleavage efficiency by the Kaposi's sarcoma-associated herpesvirus endonuclease SOX

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About: Site specific target binding controls RNA cleavage efficiency by the Kaposi's sarcoma-associated herpesvirus endonuclease SOX Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Site specific target binding controls RNA cleavage efficiency by the Kaposi's sarcoma-associated herpesvirus endonuclease SOX
Creator	Bohne, Jens Vogt, Carolin Glaunsinger, Britt Mendez, Aaron
Source	Medline; PMC
abstract	A number of viruses remodel the cellular gene expression landscape by globally accelerating messenger RNA (mRNA) degradation. Unlike the mammalian basal mRNA decay enzymes, which largely target mRNA from the 5′ and 3′ end, viruses instead use endonucleases that cleave their targets internally. This is hypothesized to more rapidly inactivate mRNA while maintaining selective power, potentially though the use of a targeting motif(s). Yet, how mRNA endonuclease specificity is achieved in mammalian cells remains largely unresolved. Here, we reveal key features underlying the biochemical mechanism of target recognition and cleavage by the SOX endonuclease encoded by Kaposi's sarcoma-associated herpesvirus (KSHV). Using purified KSHV SOX protein, we reconstituted the cleavage reaction in vitro and reveal that SOX displays robust, sequence-specific RNA binding to residues proximal to the cleavage site, which must be presented in a particular structural context. The strength of SOX binding dictates cleavage efficiency, providing an explanation for the breadth of mRNA susceptibility observed in cells. Importantly, we establish that cleavage site specificity does not require additional cellular cofactors, as had been previously proposed. Thus, viral endonucleases may use a combination of RNA sequence and structure to capture a broad set of mRNA targets while still preserving selectivity.
has issue date	2018-12-14(xsd:dateTime)
bibo:doi	10.1093/nar/gky932
bibo:pmid	30321376
has license	cc-by
sha1sum (hex)	bfaeecd7b15a7d6d0563cd323c40681e285d70e7
schema:url	https://doi.org/10.1093/nar/gky932
resource representing a document's title	Site specific target binding controls RNA cleavage efficiency by the Kaposi's sarcoma-associated herpesvirus endonuclease SOX
has PubMed Central identifier	PMC6294519
has PubMed identifier	30321376
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:bfaeecd7b15a7d6d0563cd323c40681e285d70e7#body_text
is schema:about of	named entity 'decay' named entity 'mRNA' named entity 'TARGET' named entity 'ASSOCIATED' named entity 'CLEAVAGE' named entity 'SOX' named entity 'CELLULAR' named entity 'SARCOMA' named entity 'FEATURES' named entity 'HERPESVIRUS' named entity 'KSHV' named entity 'SITE' named entity 'MOTIF' named entity 'CELLS' named entity 'SITE SPECIFICITY' named entity 'ROBUST' named entity 'THEIR' named entity 'RNA SEQUENCE' named entity 'PROXIMAL TO' named entity 'ENDONUCLEASE' named entity 'CAPTURE' named entity 'MESSENGER RNA' named entity 'TARGET' named entity 'RECONSTITUTED' named entity 'PRESENTED' named entity 'PREVIOUSLY' named entity 'PROPOSED' named entity 'LANDSCAPE' named entity 'SPECIFIC' named entity 'DOES NOT' named entity 'KEY' named entity 'BIOCHEMICAL MECHANISM' named entity 'ENDONUCLEASES' named entity 'MRNA' named entity 'OBSERVED' named entity 'USE' named entity 'VIRUSES' named entity 'CLEAVAGE REACTION' named entity '28S' named entity 'RAPIDLY' named entity 'ENCODED' named entity 'SPECIFICITY' named entity 'STRENGTH' named entity 'HOW' named entity 'BREADTH' named entity 'GENE EXPRESSION' named entity 'MAMMALIAN' named entity 'ENDONUCLEASE' named entity 'GLOBALLY' named entity 'SET' named entity 'IN VITRO' named entity 'TARGETING' named entity 'POWER' named entity 'REVEAL' named entity 'LARGELY' named entity 'PRESERVING' named entity 'PURIFIED' named entity 'USING' named entity 'END' named entity 'STRUCTURAL' named entity 'RNA CLEAVAGE' named entity 'CONTEXT' named entity 'RECOGNITION' named entity '27S' named entity 'BASAL' named entity 'HERPESVIRUS' named entity 'SARCOMA' named entity 'SOX' named entity '27S' named entity 'KAPOSI' named entity 'BINDING' named entity 'HERE' named entity 'PROVIDING' named entity 'DEGRADATION' named entity 'RNA BINDING' named entity 'STRUCTURE' named entity 'INTERNALLY' named entity 'COFACTORS'

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