About: Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain

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About: Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain
Creator	Agric, Front,
Source	Medline; PMC
abstract	The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.
has issue date	2007-01-01(xsd:dateTime)
bibo:doi	10.1007/s11703-007-0060-5
bibo:pmid	32214985
has license	no-cc
sha1sum (hex)	bf3d93543f293e133e608e231b6ae0999e73b654
schema:url	https://doi.org/10.1007/s11703-007-0060-5
resource representing a document's title	Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain
has PubMed Central identifier	PMC7088836
has PubMed identifier	32214985
schema:publication	Front Agric China
resource representing a document's body	covid:bf3d93543f293e133e608e231b6ae0999e73b654#body_text
is schema:about of	named entity 'gene' named entity 'RT-PCR' named entity 'antibody' named entity 'nucleocapsid' named entity 'gastroenteritis' named entity 'TGEV' named entity 'SEQUENCE COMPARISON' named entity 'NAMED' named entity 'AMPLIFIED' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'RECOMBINANT' named entity 'PROTEIN GENE' named entity 'REACTIONS' covid:arg/bf3d93543f293e133e608e231b6ae0999e73b654 named entity 'STRAIN' named entity 'COMPLETE' named entity 'LENGTH' named entity 'PETN' named entity 'SELECTED' named entity 'PROKARYOTIC EXPRESSION' named entity 'EXPRESSION VECTOR' named entity 'ECORI' named entity 'SHARES' named entity 'SEQUENCE' named entity 'PROTEIN GENE' named entity 'SPECIFIC ANTIBODY' named entity 'EXPRESSED' named entity 'ISOPROPYL' named entity 'WESTERN' named entity 'SDS-PAGE' named entity 'SITES' named entity 'GENE BANK' named entity 'STRONG POSITIVE' named entity 'MORE THAN' named entity 'PET' named entity 'PROTEIN ' named entity 'HOMOLOGY' named entity 'CLONING' named entity 'NUCLEOCAPSID PROTEIN' named entity 'REVEALED' named entity 'INDUCED' named entity '149' named entity 'NUCLEOTIDE' named entity 'KDA' named entity 'N GENE' named entity 'MULTIPLE' named entity 'EXPRESSION' named entity 'RT-PCR' named entity 'ISOLATED STRAIN' named entity 'NUCLEOCAPSID PROTEIN' named entity 'CLONING' named entity 'STRAINS' named entity 'CLONED' named entity 'RESULT' named entity 'BLOT' named entity 'BEING' named entity 'recombinant' named entity 'transmissible' named entity 'amplified' named entity 'induced' named entity 'nucleocapsid' named entity 'gene' named entity 'antibody' named entity 'EcoRI' named entity 'transmissible gastroenteritis virus' named entity 'RT-PCR' named entity 'Western-blot' named entity 'SDS-PAGE' named entity 'gene' named entity 'gene' named entity 'reactions' named entity 'ORF' named entity 'gene' named entity 'centrifugation' named entity 'Coomassie blue' named entity 'gene' named entity 'Western blot' named entity 'sense and antisense' named entity 'immunoglobulin' named entity 'Reverse transcription'

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