About: Method for accurate diagnose of lupus erythematosus skin lesions based on microbial rDNA sequencing

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Method for accurate diagnose of lupus erythematosus skin lesions based on microbial rDNA sequencing
Creator	Liu, Chunyan Zhang, Bin Zhao, Ying Ren, Guohua
Source	Medline; PMC
abstract	This study used microbial rDNA sequencing for accurate detection of systemic lupus erythematosus (SLE) skin lesions. 20 lupus erythematosus dermatology patients and 20 healthy persons were selected as experimental group and control group. Feces and serum of the subjects were sampled in sterile environment. Serum samples were examined for anti dsDNA antibodies. Fecal samples were analyzed by 16 s rDNA high-throughput sequencing, using Illumina MiSeq 2 × 250 sequencing platform. The results suggested that positive rate of anti-dsDNA antibody in serum was significantly higher in the experimental group than the control group. Significant difference of intestinal microbiome was spotted between the two groups in phylum (Firmicutes, Bacteroidetes) and genus level (Lactobacillus, Allobaculum, Lachnospira, Turicibacter, Bifidobacterium). The different intestinal microbiomes existing in healthy people and patients can provide a strong tool for accurate diagnose of lupus erythematosus.
has issue date	2020-05-28(xsd:dateTime)
bibo:doi	10.1016/j.sjbs.2020.05.035
bibo:pmid	32714036
has license	cc-by-nc-nd
sha1sum (hex)	be2268f17ad00c20874fa4ee03532069c5692d8d
schema:url	https://doi.org/10.1016/j.sjbs.2020.05.035
resource representing a document's title	Method for accurate diagnose of lupus erythematosus skin lesions based on microbial rDNA sequencing
has PubMed Central identifier	PMC7376186
has PubMed identifier	32714036
schema:publication	Saudi J Biol Sci
resource representing a document's body	covid:be2268f17ad00c20874fa4ee03532069c5692d8d#body_text
is schema:about of	named entity 'sequencing' named entity 'positive' named entity 'antibodies' named entity 'skin lesions' named entity 'SAMPLED' named entity 'subjects' named entity 'accurate' named entity 'phylum' named entity 'rDNA' named entity 'sterile' named entity 'environment' named entity 'accurate' named entity 'lupus' named entity 'Firmicutes' named entity 'suggested' named entity 'Feces' named entity 'dermatology' named entity 'antibody' named entity 'microbial' named entity 'anti-dsDNA' named entity 'antibody' named entity 'Allobaculum' named entity 'serum' named entity 'serum' named entity 'high-throughput sequencing' named entity 'systemic lupus erythematosus' named entity 'SLE' named entity 'intestinal microbiome' named entity 'dsDNA' named entity 'lupus erythematosus' named entity 'Lactobacillus' named entity 'microbial' named entity 'rDNA' named entity 'by-nc-nd' named entity 'DNA library' named entity 'Lachnospiraceae' named entity 'cerebral vasculitis' named entity 'anti-dsDNA' named entity 'statistical analysis' named entity 'Simpson index' named entity 't-test' named entity 'electrophoresis' named entity 'phylum' named entity 'Survival rate' named entity 'gene expression profiling' named entity 'experimental group' named entity 'infection' named entity 'Lachnospiraceae' named entity 'normal distribution' named entity 'phylogenesis' named entity 'phylum' named entity 'fetus' named entity 'survival rate' named entity 'Intestinal microbiome' named entity 'hormone' named entity 'bioinformatical' named entity 'severe pneumonia' named entity 'statistically significant' named entity 'intestinal microbes' named entity 'microbiome' named entity 'clinical research' named entity 'hormones' named entity 'fecal' named entity 'agarose' named entity 'genus' named entity 'enzyme' named entity 'SPSS' named entity 'nephrotic syndrome' named entity 'Discoid' named entity 'Q-Q' named entity 'hypertensive encephalopathy' named entity 'DNA library' named entity 'severe pneumonia' named entity 'Proteobacteria' named entity 'Jiangsu' named entity 'skin lesions' named entity 'control group' named entity 'diabetes'

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