About: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells

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About: Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
Creator	Zhang, Jun Ma, Jian Yuan, Quan Li, Zhi-Yong Yang, Ting Ge, Sheng-Xiang Hou, Wang-Heng Xia, Ning-Shao Yao, Xiang-Yang Zhang, Ya-Li Cao, Jia-Li Qiao, Xiao-Yang Xu, Jing-Jing Xiong, Hua-Long Yang, Ren Zhang, Tian-Ying Author, † Fu, Bao-Rong Huang, Bao-Ying Liang-Zhang, Wang, Shao-Juan Wu, Yang-Tao Zhang, Bao-Hui
source	BioRxiv
abstract	The global pandemic of Coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable in vitro neutralization assay is very important for the development of neutralizing antibodies, vaccines and other inhibitors. In this study, G protein-deficient vesicular stomatitis virus (VSVdG) bearing full-length and truncated spike (S) protein of SARS-CoV-2 were evaluated. The virus packaging efficiency of VSV-SARS-CoV-2-Sdel18 (S with C-terminal 18 amino acid truncation) is much higher than VSV-SARS-CoV-2-S. A neutralization assay for antibody screening and serum neutralizing titer quantification was established based on VSV-SARS-CoV-2-Sdel18 pseudovirus and human angiotensin-converting enzyme 2 (ACE2) overexpressed BHK21 cell (BHK21-hACE2). The experimental results can be obtained by automatically counting EGFP positive cell number at 12 hours after infection, making the assay convenient and high-throughput. The serum neutralizing titer of COVID-19 convalescent patients measured by VSV-SARS-CoV-2-Sdel18 pseudovirus assay has a good correlation with live SARS-CoV-2 assay. Seven neutralizing monoclonal antibodies targeting receptor binding domain (RBD) of SARS-CoV-2-S were obtained. This efficient and reliable pseudovirus assay model could facilitate the development of new drugs and vaccines.
has issue date	2020-04-13(xsd:dateTime)
bibo:doi	10.1101/2020.04.08.026948
has license	biorxiv
sha1sum (hex)	bda5dbec80bd7051a00c53767a833556e96fa1ab
schema:url	https://doi.org/10.1101/2020.04.08.026948
resource representing a document's title	Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
schema:publication	bioRxiv
resource representing a document's body	covid:bda5dbec80bd7051a00c53767a833556e96fa1ab#body_text
is schema:about of	named entity 'number' named entity 'neutralizing' named entity 'Coronavirus disease 2019' named entity 'development' named entity 'POSITIVE CELL' named entity 'NEUTRALIZING ANTIBODIES' named entity 'AMINO ACID' named entity 'LENGTH' named entity 'SARS-COV-2' named entity 'PANDEMIC' named entity 'INFECTION' named entity 'DISASTER' named entity 'EFFICIENT' named entity 'AUTOMATICALLY' named entity 'human society' named entity 'screening' named entity 'reliable' named entity 'assay' named entity 'evaluated' named entity 'good' named entity 'vesicular stomatitis virus' named entity 'overexpressed' named entity 'neutralizing antibodies' named entity 'SARS-CoV-2' named entity 'neutralizing' named entity 'quantification' named entity 'VSV' named entity 'virus' named entity 'serum' named entity 'antibody' named entity 'vesicular stomatitis virus' named entity 'pseudotyped' named entity 'pseudotyped' named entity 'angiotensin-converting enzyme 2' named entity 'SARS-CoV-2' named entity 'protein' named entity 'SARS-CoV-2' named entity 'plasma membrane' named entity 'cloned' named entity 'high-throughput' named entity 'VSV' named entity 'hybridoma' named entity 'cell surface' named entity 'protein' named entity 'serum' named entity 'SARS-CoV-2' named entity 'lipid bilayer' named entity 'Retrovirus' named entity 'C-terminal' named entity 'viruses' named entity 'codon' named entity 'C-terminal' named entity 'spike protein' named entity 'spike protein' named entity 'VSV' named entity 'VSV' named entity 'VSV' named entity 'hACE2' named entity 'VSV' named entity 'pseudovirus' named entity 'titer' named entity 'IC50'

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