About: M and N proteins of SARS coronavirus induce apoptosis in HPF cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	M and N proteins of SARS coronavirus induce apoptosis in HPF cells
Creator	Yang, Ying Peng, Jing-Pian Shi, Shu-Qun Zhao, Gang
Source	Medline; PMC
abstract	Background: SARS-associated coronavirus (SARS-CoV) induced cell apoptosis and its structural proteins may play a role in this process. Objectives: To determine whether the structural proteins M and N of SARS-CoV induce apoptosis. Study design: We investigated human pulmonary fibroblast (HPF) cells, were transfected with plasmids containing the M or N gene, by TdT-mediated dUTP nick end labeling (TUNEL), Hoechst 33342 staining for nuclei, and observation of morphology. Results: We found that in the absence of serum about 16.34% of cells transfected by pcDNA3.1-M and 21.72% of N-transfected cells showed typical apoptotic characteristics, significantly different from mock-transfected cells (only 6.23%, p<0.01). Furthermore, the cells that were co-transfected with M and N proteins showed more obvious phenomena of cell death (about 36.03%). There was a statistical significance between M-transfected cells and co-transfected cells (p<0.01), and a remarkable difference between N-transfected cells and co-transfected cells (p<0.01). Conclusions: The results show that M and N proteins of SARS-CoV can induce apoptosis of HPF cells. Co-transfection of M and N enhances the induction of apoptosis by M or N alone, which also suggests that the structural proteins of SARS-CoV may play an important role not only in the process of invasion but also in the pathogenetic process in cells.
has issue date	2006-07-16(xsd:dateTime)
bibo:doi	10.1007/s10565-006-0077-1
bibo:pmid	16845612
has license	no-cc
sha1sum (hex)	bd996a0dd036e9453e23bef84300b51ffe0524ee
schema:url	https://doi.org/10.1007/s10565-006-0077-1
resource representing a document's title	M and N proteins of SARS coronavirus induce apoptosis in HPF cells
has PubMed Central identifier	PMC7087820
has PubMed identifier	16845612
schema:publication	Cell Biol Toxicol
resource representing a document's body	covid:bd996a0dd036e9453e23bef84300b51ffe0524ee#body_text
is schema:about of	named entity 'investigated' named entity 'HPF' named entity 'obvious' named entity 'induced' covid:arg/bd996a0dd036e9453e23bef84300b51ffe0524ee named entity 'process' named entity 'SARS-associated coronavirus' named entity 'apoptotic' named entity 'structural proteins' named entity 'process' named entity 'apoptosis' named entity 'gene' named entity 'statistical significance' named entity 'nuclei' named entity 'transfected' named entity 'transfected' named entity '0.01' named entity 'SARS-associated coronavirus' named entity 'apoptosis' named entity 'serum' named entity 'virus' named entity 'protein' named entity 'sodium citrate' named entity 'ANOVA' named entity 'protein' named entity 'TUNEL' named entity 'Jurkat' named entity 'Beta-actin' named entity 'SARS-CoV' named entity 'cell death' named entity 'plasmids' named entity 'SARS-CoV' named entity 'serum' named entity 'SARS' named entity 'paraformaldehyde' named entity 'transfection' named entity 'DMEM' named entity 'SARS-CoV' named entity 'FITC' named entity 'propidium iodide' named entity 'PBS' named entity 'SARS-CoV' named entity 'paraformaldehyde' named entity 'cell lines' named entity 'Hoechst' named entity 'nuclei' named entity 'fluorescein isothiocyanate' named entity 'nuclei' named entity 'SARS' named entity 'light microscope' named entity 'transfection' named entity 'lungs' named entity 'transfected' named entity 'Incubate' named entity 'biochemical' named entity 'SARS' named entity 'Hoechst' named entity 'DMEM' named entity 'Lipofectamine' named entity 'pathogen' named entity 'Bcl-xL' named entity 'PBS' named entity 'apoptosis' named entity 'intracellular membrane' named entity 'SARS-CoV' named entity 'apoptosis' named entity 'serum' named entity 'fluorescence' named entity 'penicillin' named entity 'apoptosis' named entity 'DMEM' named entity 'transfection' named entity 'SARS virus' named entity 'dye' named entity 'Invitrogen' named entity 'pathogenetic' named entity 'apoptosis' named entity 'staining'

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