About: An alternative −1/+2 open reading frame exists within viral Npro(1–19) region of bovine viral diarrhea virus SD-1

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About: An alternative −1/+2 open reading frame exists within viral Npro(1–19) region of bovine viral diarrhea virus SD-1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An alternative −1/+2 open reading frame exists within viral Npro(1–19) region of bovine viral diarrhea virus SD-1
Creator	Fan, Zhen-Chuan Curtis Bird, R
Source	Elsevier; Medline; PMC
abstract	Abstract We previously reported the engineering of an Npro-disrupted bovine viral diarrhea virus (BVDV), BSD1-Npro/eGFP2A (Fan and Bird, 2008a). Here, we report that BSD1-Npro/eGFP2A survives a single nucleotide missing in its C-terminal eGFP region. By using our established reverse genetics system for BVDV, we confirm that the viral mutant is rescued through a −1/+2 ORF initiated in the Npro(1–19)/eGFP region of the mutant viral genome. We furthermore uncover that this event occurs in the Npro(1–19) region of BVDV strain SD-1. The rescued viral mutant showed dramatic reductions in levels of both viral RNA and viral protein in host cells. Although the mutant is similar to the native strain in viral kinetics, the peak yield of the mutant is decreased dramatically. These findings reveal the existence of an alternative −1/+2 ORF in the Npro(1–19) region during the replication of BVDV and open a new avenue to understand the life cycle and pathogenesis of pestiviruses.
has issue date	2012-01-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2011.10.022
bibo:pmid	22079882
has license	els-covid
sha1sum (hex)	bd823fe395aa4e12c4af2521f7bb334e61245beb
schema:url	https://doi.org/10.1016/j.virusres.2011.10.022
resource representing a document's title	An alternative −1/+2 open reading frame exists within viral Npro(1–19) region of bovine viral diarrhea virus SD-1
has PubMed Central identifier	PMC7172404
has PubMed identifier	22079882
schema:publication	Virus Research
resource representing a document's body	covid:bd823fe395aa4e12c4af2521f7bb334e61245beb#body_text
is schema:about of	named entity 'These' named entity 'BVDV' named entity 'system' named entity 'replication' named entity 'viral' named entity 'viral' named entity 'open reading frame' named entity 'THE PEAK' named entity 'DISRUPTED' named entity 'REPLICATION' named entity 'REPORTED' named entity 'REPORT' named entity 'C-TERMINAL' named entity 'SINGLE' named entity 'PRO' named entity 'NATIVE' named entity 'pro' named entity 'pro' named entity 'genome' named entity 'bovine viral diarrhea' named entity 'mutant' named entity 'BVDV' named entity 'RNA' named entity 'kinetics' named entity 'mutant' named entity 'survives' named entity 'dramatic' named entity 'alternative' named entity 'ORF' named entity 'eGFP' named entity 'pathogenesis' named entity 'BVDV' named entity 'ORF' named entity 'reverse genetics' named entity 'BVDV' named entity 'pathogenesis' named entity 'confirm' named entity 'IgG' named entity 'FFU' named entity 'frameshift' named entity 'La Rocca' named entity 'viruses' named entity 'western blotting' named entity 'growth kinetics' named entity 'viruses' named entity 'real-time RT-PCR' named entity 'FMDV' named entity 'ribosome' named entity 'ribosome' named entity 'RNA' named entity 'incubation' named entity 'frameshift' named entity 'viral RNA' named entity 'ORF' named entity 'BVDV' named entity 'cysteine' named entity 'cDNA' named entity 'viral genomes' named entity 'RNAs' named entity 'green fluorescent protein' named entity 'MOI' named entity 'RT-PCR' named entity 'coding region' named entity 'RNA genome' named entity 'virus' named entity 'viral RNA' named entity 'genome' named entity 'fluorescence' named entity 'E2 protein' named entity 'GFP' named entity 'GFP' named entity 'antibodies' named entity 'coding region' named entity 'BVDV' named entity 'FMDV' named entity 'ribosome' named entity 'viral assembly' named entity 'ORF' named entity 'BVDV' named entity 'freeze-thaw'

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