About: Basic insights into Zika virus infection of neuroglial and brain endothelial cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Basic insights into Zika virus infection of neuroglial and brain endothelial cells
Creator	Mahalingam, Suresh Merits, Andres Liu, Xiang King, Nicholas Burt, Felicity Grau, Georges Ekberg, Jenny Hueston, Linda Ling, Zheng Mutso, Margit Poo, Yee St, James
source	Medline; PMC
abstract	Zika virus (ZIKV) has recently emerged as an important human pathogen due to the strong evidence that it causes disease of the central nervous system, particularly microcephaly and Guillain–Barré syndrome. The pathogenesis of disease, including mechanisms of neuroinvasion, may include both invasion via the blood–brain barrier and via peripheral (including cranial) nerves. Cellular responses to infection are also poorly understood. This study characterizes the in vitro infection of laboratory-adapted ZIKV African MR766 and two Asian strains of (1) brain endothelial cells (hCMEC/D3 cell line) and (2) olfactory ensheathing cells (OECs) (the neuroglia populating cranial nerve I and the olfactory bulb; both human and mouse OEC lines) in comparison to kidney epithelial cells (Vero cells, in which ZIKV infection is well characterized). Readouts included infection kinetics, intracellular virus localization, viral persistence and cytokine responses. Although not as high as in Vero cells, viral titres exceeded 10(4) plaque-forming units (p.f.u.) ml(−1) in the endothelial/neuroglial cell types, except hOECs. Despite these substantial titres, a relatively small proportion of neuroglial cells were primarily infected. Immunolabelling of infected cells revealed localization of the ZIKV envelope and NS3 proteins in the cytoplasm; NS3 staining overlapped with that of dsRNA replication intermediate and the endoplasmic reticulum (ER). Infected OECs and endothelial cells produced high levels of pro-inflammatory chemokines. Nevertheless, ZIKV was also able to establish persistent infection in hOEC and hCMEC/D3 cells. Taken together, these results provide basic insights into ZIKV infection of endothelial and neuroglial cells and will form the basis for further study of ZIKV disease mechanisms.
has issue date	2020-04-30(xsd:dateTime)
bibo:doi	10.1099/jgv.0.001416
bibo:pmid	32375993
has license	cc-by
sha1sum (hex)	bbbe6c93c701ddee69d2571e2bcacc1528875b7e
schema:url	https://doi.org/10.1099/jgv.0.001416
resource representing a document's title	Basic insights into Zika virus infection of neuroglial and brain endothelial cells
has PubMed Central identifier	PMC7414445
has PubMed identifier	32375993
schema:publication	J Gen Virol
resource representing a document's body	covid:bbbe6c93c701ddee69d2571e2bcacc1528875b7e#body_text
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