About: Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus

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About: Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
Creator	Sridhar, Siddharth Yuen, Kwok-Yung Chan, Kwok-Hung Lam, Ho-Yin Cheng, Andrew Yip, Cyril Leung, Kit-Hang Chan, Jasper Cheng, Vincent Fung, Ami Tang, Bone Wong, Samson
Source	Medline; PMC
abstract	BACKGROUND: Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system. METHODS: The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. RESULTS: Linear regression analysis of the quantitative results by the in-house assay showed the dynamic range from 2.60 to 9 log(10) (plasma) and 2.94 to 9 log(10) (viral transport medium) copies/mL, with the coefficient of determination (R(2)) of 0.996 and 0.998, respectively. A dilution series demonstrated the limits of detection and lower limits of quantification for plasma were 2.06 log(10) and 2.60 log(10) copies/mL and those for viral transport medium were 2.31 log(10) and 2.94 log(10) copies/mL respectively. The precision of the in-house assay was highly reproducible among runs with coefficients of variance ranging from 0.07 to 3.21% for plasma and 0.17% to 2.11% for viral transport medium. A comparison of 52 matched samples showed an excellent correlation between the quantitative viral loads measured by the in-house assay and the RealStar® Adenovirus PCR Kit (R(2) = 0.984), with an average bias of − 0.16 log(10) copies/mL. CONCLUSIONS: The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1059-7) contains supplementary material, which is available to authorized users.
has issue date	2018-09-27(xsd:dateTime)
bibo:doi	10.1186/s12985-018-1059-7
bibo:pmid	30261891
has license	cc-by
sha1sum (hex)	bb6d4ff2bd0aa48e1d8b2b18f937a244cd806212
schema:url	https://doi.org/10.1186/s12985-018-1059-7
resource representing a document's title	Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
has PubMed Central identifier	PMC6161464
has PubMed identifier	30261891
schema:publication	Virol J
resource representing a document's body	covid:bb6d4ff2bd0aa48e1d8b2b18f937a244cd806212#body_text
is schema:about of	named entity 'PCR ASSAY' named entity 'coefficients' named entity 'community-acquired' named entity 'analytical' named entity 'log' named entity 'infections' named entity 'Adenovirus' named entity 'determination' named entity 'plasma' named entity 'Adenovirus' named entity 'copies' named entity 'quantitative' named entity 'mortality rates' named entity 'plasma' named entity 'PCR' named entity 'assay' named entity 'adenovirus' named entity 'KIT' named entity 'COMPARATIVE EVALUATION' named entity 'LOWER' named entity 'LIMITS OF DETECTION' named entity 'HUMAN' named entity 'CORRELATION' named entity 'VIRAL TRANSPORT MEDIUM' named entity 'POLYMERASE CHAIN REACTION ASSAY' named entity 'RESPONSE TO' named entity 'RUNS' named entity 'BIAS' named entity 'TRANSPORT MEDIUM' named entity 'DYNAMIC RANGE' named entity 'CONCLUSIONS' named entity 'EXCELLENT' named entity 'SPECIFICITY' named entity 'MORTALITY RATES' named entity 'INFECTIONS' named entity 'VIRAL' named entity 'COMMERCIAL' named entity 'KIT' named entity 'PRECISION' named entity 'DETECTION' named entity 'MAY BE' named entity 'IN-HOUSE' named entity 'VARIANCE' named entity 'IS A' named entity 'DNA' named entity 'PCR' named entity 'LINEAR REGRESSION ANALYSIS' named entity 'EVALUATED' named entity 'EFFECTIVE' named entity '0.16' named entity 'RESULTS' named entity 'SERIES' named entity 'ANTIVIRAL TREATMENT' named entity 'AVERAGE' named entity 'ADENOVIRUS' named entity 'QUANTIFICATION' named entity 'LIMITS' named entity 'GERMANY' named entity 'COMMON' named entity 'BUT' named entity 'LOG' named entity 'COMPARISON' named entity 'USING' named entity 'QUANTITATIVE' named entity 'PERFORMANCE' named entity 'PROFICIENCY TESTING' named entity 'HAMBURG' named entity 'COMPARED' named entity 'LABORATORY' named entity 'BACKGROUND'

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