About: A simple microbiome in the European common cuttlefish, Sepia officinalis

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About: A simple microbiome in the European common cuttlefish, Sepia officinalis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A simple microbiome in the European common cuttlefish, Sepia officinalis
Creator	Gilbert, Jack Abbo, Lisa Durand, Amber Gottel, Neil Hanlon, Roger Lutz, Holly Schlundt, Sjaarda, Alexandra Tabita Ramírez-Puebla, S Welch, L
Source	BioRxiv
abstract	The European common cuttlefish, Sepia officinalis, is used extensively in biological and biomedical research yet its microbiome remains poorly characterized. We analyzed the microbiota of the digestive tract, gills, and skin in mariculture-raised S. officinalis using a combination of 16S rRNA amplicon sequencing, qPCR and fluorescence spectral imaging. Sequencing revealed a highly simplified microbiota consisting largely of two single bacterial amplicon sequence variants (ASVs) of Vibrionaceae and Piscirickettsiaceae. The esophagus was dominated by a single ASV of the genus Vibrio. Imaging revealed bacteria in the family Vibrionaceae distributed in a discrete layer that lines the esophagus. This Vibrio was also the primary ASV found in the microbiota of the stomach, cecum, and intestine, but occurred at lower abundance as determined by qPCR and was found only scattered in the lumen rather than in a discrete layer via imaging analysis. Treatment of animals with the commonly-used antibiotic enrofloxacin led to a nearly 80% reduction of the dominant Vibrio ASV in the esophagus but did not significantly alter the relative abundance of bacteria overall between treated versus control animals. Data from the gills was dominated by a single ASV in the family Piscirickettsiaceae, which imaging visualized as small clusters of cells. We conclude that bacteria belonging to the Gammaproteobacteria are the major symbionts of the cuttlefish Sepia officinalis cultured from eggs in captivity, and that the esophagus and gills are major colonization sites. IMPORTANCE Microbes can play critical roles in the physiology of their animal hosts, as evidenced in cephalopods by the role of Vibrio (Aliivibrio) fischeri in the light organ of the bobtail squid and the role of Alpha- and Gammaproteobacteria in the reproductive system and egg defense in a variety of cephalopods. We sampled the cuttlefish microbiome throughout the digestive tract, gills, and skin and found dense colonization of an unexpected site, the esophagus, by a microbe of the genus Vibrio, as well as colonization of gills by Piscirickettsiaceae. This finding expands the range of organisms and body sites known to be associated with Vibrio and is of potential significance for understanding host-symbiont associations as well as for understanding and maintaining the health of cephalopods in mariculture.
has issue date	2019-03-06(xsd:dateTime)
bibo:doi	10.1101/440677
has license	biorxiv
sha1sum (hex)	baa8608f9b42c8c4069a16cdacfe3d5ac5f753c7
schema:url	https://doi.org/10.1101/440677
resource representing a document's title	A simple microbiome in the European common cuttlefish, Sepia officinalis
schema:publication	bioRxiv
resource representing a document's body	covid:baa8608f9b42c8c4069a16cdacfe3d5ac5f753c7#body_text
is schema:about of	named entity 'cuttlefish' named entity 'cephalopods' named entity 'colonization' named entity 'Vibrio' named entity 'body' named entity 'variety' named entity 'Vibrionaceae' named entity 'fluorescence in situ hybridization' named entity 'Vibrio' named entity 'mariculture' named entity 'Sepia officinalis' named entity 'fluorophore' named entity 'bacteria' named entity 'pathogens' named entity 'food source' named entity 'esophagus' named entity 'grass shrimp' named entity 'genus' named entity 'Vibrionaceae' named entity 'control group' named entity 'cultured cells' named entity 'cuttlefish' named entity 'cephalopod' named entity 'sequence data' named entity 'amplicon' named entity 'Euprymna scolopes' named entity 'antibiotic' named entity 'seawater' named entity 'stomach' named entity 't-test' named entity 'GI tracts' named entity 'internal organs' named entity 'qPCR' named entity 'lumen' named entity 'hybridized' named entity 'bacteria' named entity 'Vibrio' named entity 'epithelium' named entity 'Carl Zeiss' named entity 'GI tract' named entity 'GI tract' named entity 'cecum' named entity 'mysids' named entity 'negative control' named entity 'UniFrac' named entity 'microbiota' named entity 'Vibrio' named entity 'quantitative PCR' named entity 'physiology' named entity 'Piscirickettsiaceae' named entity 'distal' named entity 'cuttlefish' named entity 'sequence data' named entity 'Vibrio' named entity 'Vibrio' named entity 'Calanus finmarchicus' named entity 'Palaemonetes' named entity 'Vibrio' named entity 'Gammaproteobacteria' named entity 'intestine' named entity '10.8' named entity 'clams' named entity 'cuttlefish' named entity 'microbiota' named entity 'significant difference' named entity 'Piscirickettsiaceae' named entity '16S rRNA gene' named entity 'Piscirickettsiaceae' named entity 'Vibrio' named entity 'bacteria' named entity 'Loligo' named entity 'sequence variants' named entity 'Bacteria' named entity 'antibiotic treatment' named entity 'esophagus'

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