About: In silico analysis of a chimeric fusion protein as a new vaccine candidate against Clostridium perfringens type A and Clostridium septicum alpha toxins

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About: In silico analysis of a chimeric fusion protein as a new vaccine candidate against Clostridium perfringens type A and Clostridium septicum alpha toxins Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In silico analysis of a chimeric fusion protein as a new vaccine candidate against Clostridium perfringens type A and Clostridium septicum alpha toxins
Creator	Ranjbar, Mohammad Faghihloo, Ebrahim Ghalavand, Zohreh Goudarzi, Hossein Haghroosta, Ali Langroudi, Reza
Source	PMC
abstract	In silico analysis is the most important approach to understand protein structure and functions, and the most important problem for designing and producing a fusion construct is producing large amounts of functional protein. Clostridium perfringens type A and Clostridium septicum produce alpha (plc) and alpha toxins respectively. C. perfringens can cause gas gangrene and gastrointestinal diseases. C. septicum can cause traumatic and non-traumatic gas gangrene. The aim of current research was in silico analysis of a chimeric fusion protein against C. perfringens type A and C. septicum alpha toxins. Firstly, the chimeric fusion gene was designed according to nucleotide sequences of C. perfringens type A alpha (KY584046.1) and C. septicum alpha (JN793989.2) toxin genes and then its fusion protein is constructed by amino acid sequences of C. perfringens type A and C. septicum alpha toxins. Secondly, online software was used to determine prediction of secondary and tertiary structures and physicochemical characteristics of the fusion protein. Finally, the validation of the fusion protein was confirmed by Rampage and proSA program. The designed fusion protein has 777 amino acids in length. TASSER server and physicochemical parameters are showed: C-score = − 2.68 and molecular weight = 87.9 KD respectively. Rampage and proSA software revealed the fusion protein is valid. Deposited accession number for the sequence of the fusion gene in the GenBank is MK908396. The designed fusion protein is valid and functional. Thus, the fusion gene could be used for clone and expression in a proper prokaryotic cell and also as a recombinant vaccine candidate.
has issue date	2020-07-14(xsd:dateTime)
bibo:doi	10.1007/s00580-020-03136-6
has license	cc-by
sha1sum (hex)	ba51c2bf642f5b3ed67bb9dff3dcb8978f4ce034
schema:url	https://doi.org/10.1007/s00580-020-03136-6
resource representing a document's title	In silico analysis of a chimeric fusion protein as a new vaccine candidate against Clostridium perfringens type A and Clostridium septicum alpha toxins
has PubMed Central identifier	PMC7358331
schema:publication	Comp Clin Path
resource representing a document's body	covid:ba51c2bf642f5b3ed67bb9dff3dcb8978f4ce034#body_text
is schema:about of	named entity 'fusion protein' named entity 'revealed' named entity 'alpha' named entity 'valid' named entity 'validation' named entity 'tertiary structures' named entity 'accession number' named entity 'type A' named entity 'Clostridium' named entity 'alpha' named entity 'NON-' named entity 'PARAMETERS' named entity '2.68' named entity 'RECOMBINANT VACCINE' named entity 'EXPRESSION' named entity 'SCORE' named entity 'SERVER' named entity 'AIM' named entity 'VALID' named entity 'CONFIRMED BY' named entity 'ALPHA' named entity 'FUSION PROTEIN' named entity 'IMPORTANT PROBLEM' named entity 'APPROACH' named entity 'PRODUCING' named entity 'acid' named entity 'chimeric' named entity 'problem' named entity 'fusion protein' named entity 'fusion protein' named entity 'clone' named entity 'functional' named entity 'chimeric' named entity 'type A' named entity 'physicochemical' named entity 'prokaryotic cell' named entity 'septicum' named entity 'C. perfringens' named entity 'physicochemical' named entity 'protein' named entity 'amino acids' named entity 'fusion construct' named entity 'C. perfringens' named entity 'tertiary structures' named entity 'C-score' named entity 'chimeric' named entity 'Clostridium septicum' named entity 'vaccine' named entity 'In silico' named entity 'septicum' named entity 'fusion protein' named entity 'superfamily' named entity 'septicum' named entity 'beta hairpins' named entity 'alpha toxin' named entity 'Signal peptide cleavage' named entity 'phospholipase' named entity 'Gram-positive' named entity 'C. perfringens' named entity 'C-score' named entity 'expression vector' named entity 'C. perfringens' named entity 'cloned' named entity 'fusion gene' named entity 'septicum' named entity 'fusion protein' named entity 'csa' named entity 'tertiary structure' named entity 'fusion gene' named entity 'C. perfringens' named entity 'phospholipase' named entity 'fusion protein' named entity 'E. coli' named entity 'alpha toxin' named entity 'UCSF' named entity 'gas gangrene' named entity 'fusion protein' named entity 'fusion protein'

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