About: Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase

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About: Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase
Creator	Chandran, Kartik Zhang, Yuqing Peng, Tao Das, Tandrila Hang, Howard Spence, Jennifer Chen, Chengjie Correspondence, Tao Li, Yumeng Sun, Yanan Wu, Xiaojun Yuan, Xiaoqiu
Source	Elsevier; Medline; PMC
abstract	Interferon-induced transmembrane protein 3 (IFITM3) is a key interferon effector that broadly prevents infection by diverse viruses. However, the cellular factors that control IFITM3 homeostasis and antiviral activity have not been fully elucidated. Using site-specific photo-crosslinking and quantitative proteomic analysis, here we present the identification and functional characterization of VCP/p97 AAA-ATPase as a primary interaction partner of IFITM3. We show that IFITM3 ubiquitination at lysine 24 is crucial for VCP binding, trafficking, turnover, and engagement with incoming virus particles. Consistently, pharmacological inhibition of VCP/p97 ATPase activity leads to defective IFITM3 lysosomal sorting, turnover, and co-trafficking with virus particles. Our results showcase the utility of site-specific protein photo-crosslinking in mammalian cells and reveal VCP/p97 as a key cellular factor involved in IFITM3 trafficking and homeostasis.
has issue date	2020-04-02(xsd:dateTime)
bibo:doi	10.1016/j.chembiol.2020.03.004
bibo:pmid	32243810
has license	no-cc
sha1sum (hex)	b82a213e17b6e85dc534a8a4cccab82e7d15d067
schema:url	https://doi.org/10.1016/j.chembiol.2020.03.004
resource representing a document's title	Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase
has PubMed Central identifier	PMC7194980
has PubMed identifier	32243810
schema:publication	Cell Chem Biol
resource representing a document's body	covid:b82a213e17b6e85dc534a8a4cccab82e7d15d067#body_text
is schema:about of	named entity 'Proteomics' named entity 'ATPase' named entity 'INTERACTING' named entity 'PHOTO' named entity 'ATPASE' named entity 'SPECIFIC' named entity 'leads' named entity 'Highlights' named entity 'turnover' named entity 'Turnover' named entity 'ATPase' named entity 'ubiquitination' named entity 'protein' named entity 'IFITM3' named entity 'Proteomics' named entity 'VCP' named entity 'p97' named entity 'N-terminal' named entity 'cycloalkene' named entity 'Ifitm3' named entity 'proximal' named entity 'IAV' named entity 'lysine' named entity 'IFITM3' named entity 'ubiquitinated' named entity 'virus' named entity 'plasmid' named entity 'IFITM3' named entity 'DMEM' named entity 'live cells' named entity 'ubiquitinated' named entity 'IAV' named entity '0.80' named entity 'IFITM3' named entity 'turnover rate' named entity 'downregulation' named entity 'HeLa' named entity 'proteasome' named entity 'innate immune response' named entity 'crosslinking' named entity 'crosslinking' named entity 'lysosome' named entity 'IFITM3' named entity 'oligomers' named entity 'mammalian cells' named entity '0.01' named entity 'antibody' named entity 'IFITM3' named entity 'Sigma-Aldrich' named entity 'protein' named entity 'virus replication' named entity 'anti-Flag' named entity 'cysteine' named entity 'protein' named entity 'unnatural amino acid' named entity 'co-immunoprecipitated' named entity 'alanine' named entity 'IFITM3' named entity 'steady state' named entity 'Student's t test' named entity 'covalent' named entity 'Antiviral activity' named entity 'IFITM3' named entity 'stress conditions' named entity 'Sigma-Aldrich' named entity 'pharmacological' named entity 'protein modification' named entity 'crosslinked' named entity 'IFITM3' named entity 'IFITM3'

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