About: Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus

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About: Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus
Creator	Liu, Jing Ye, Li Ye, Linbai Zhu, Ying Zheng, Yi Zeng, Yingchun Gao, Jingrong Liao, Qingjiao Lingbao, Kong Yang, Xiaojun Timani, Khalid
Source	Elsevier; Medline; PMC
abstract	Abstract A novel coronavirus (CoV) has recently been identified as the aetiological agent of severe acute respiratory syndrome (SARS). Nucleocapsid (N) proteins of the Coronaviridae family have no discernable homology, but they share a common nucleolar-cytoplasmic distribution pattern. There are three putative nuclear localization signal (NLS) motifs present in the N. To determine the role of these putative NLSs in the intracellular localization of the SARS–CoV N, we performed a confocal microscopy analysis using rabbit anti-N antisera. In this report, we show that the wild type N was distributed mainly in the cytoplasm. The N-terminal of the N, which contains the NLS1 (aa38–44), was localized to the nucleus. The C-terminus of the N, which contains both NLS2 (aa257–265) and NLS3 (aa369–390) was localized to the cytoplasm and the nucleolus. Results derived from analysis of various deletion mutations show that the region containing amino acids 226–289 is able to mediate nucleolar localization. The deletion of two hydrophobic regions that flanked the NLS3 recovered its activity and localized to the nucleus. Furthermore, deletion of leucine rich region (220-LALLLLDRLNRL) resulted in the accumulation of N to the cytoplasm and nucleolus, and when fusing this peptide to EGFP localization was cytoplasmic, suggesting that the N may act as a shuttle protein. Differences in nuclear/nucleolar localization properties of N from other members of coronavirus family suggest a unique function for N, which may play an important role in the pathogenesis of SARS.
has issue date	2005-12-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2005.05.007
bibo:pmid	15992957
has license	els-covid
sha1sum (hex)	b7bd3cb161196f1fa58ecd22d44f566d2fa45f0d
schema:url	https://doi.org/10.1016/j.virusres.2005.05.007
resource representing a document's title	Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus
has PubMed Central identifier	PMC7114095
has PubMed identifier	15992957
schema:publication	Virus Research
resource representing a document's body	covid:b7bd3cb161196f1fa58ecd22d44f566d2fa45f0d#body_text
is schema:about of	named entity 'nucleus' named entity 'deletion' named entity 'C-terminus' named entity 'ACCUMULATION' named entity 'HOMOLOGY' named entity 'NUCLEUS' named entity 'DERIVED' named entity 'CYTOPLASM' named entity 'SHARE' named entity 'NUCLEAR' named entity 'ACT' named entity 'HYDROPHOBIC' named entity 'LOCALIZED' named entity 'LOCALIZATION' named entity 'ANTISERA' named entity 'A COMMON' named entity 'AGENT' named entity 'FUNCTION' named entity 'CONTAINS' named entity 'USING' named entity 'PROTEIN' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'DISTRIBUTION PATTERN' named entity 'RABBIT' named entity 'PROPERTIES' named entity 'VARIOUS' named entity 'PROTEINS' named entity 'C-TERMINAL' named entity 'SARS CORONAVIRUS' named entity 'NUCLEOCAPSID PROTEIN' named entity 'LOCALIZATION' named entity 'AMINO ACIDS' named entity 'IDENTIFIED' named entity 'PATHOGENESIS' named entity 'NLS1' named entity 'CORONAVIRIDAE' named entity 'CONFOCAL MICROSCOPY' named entity 'FUSING' named entity 'MOTIFS' named entity 'CORONAVIRUS' named entity 'INTRACELLULAR LOCALIZATION' named entity 'IMPORTANT' named entity 'FAMILY' named entity 'DELETION' named entity 'NUCLEAR' named entity 'NUCLEAR LOCALIZATION SIGNAL' named entity 'NLS2' named entity 'NOVEL CORONAVIRUS' named entity 'REGION' named entity 'N-TERMINAL ' named entity 'FLANKED' named entity 'PROTEIN ' named entity 'DETERMINE' named entity 'CYTOPLASMIC' named entity 'MEDIATE' named entity 'UNIQUE' named entity 'NUCLEOLUS' named entity 'WILD TYPE' named entity 'ANALYSIS' named entity 'REPORT' named entity 'ROLE' named entity 'SHUTTLE' named entity 'MEMBERS' named entity 'RICH' named entity 'LEUCINE' named entity 'BUT' named entity 'ITS' named entity 'SUGGEST' named entity 'SARS' named entity 'PEPTIDE' named entity 'SARS-COV'

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