About: Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63

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About: Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63
Creator	Lin, Cheng-Wen Huang, Su-Hua Yen, Hung-Rong Lin, Yu-Ping Tsai, Yu-Chi Lee, Chia-Lin Chang, Young-Sheng
Source	Medline; PMC
abstract	Strobilanthes cusia (Nees) Kuntze is a Chinese herbal medicine used in the treatment of respiratory virus infections. The methanol extract of S. cusia leaf contains chemical components such as β-sitosterol, indirubin, tryptanthrin, betulin, indigodole A, and indigodole B that have diverse biological activities. However, the antiviral action of S. cusia leaf and its components against human coronavirus remains to be elucidated. Human coronavirus NL63 infection is frequent among immunocompromised individuals, young children, and in the elderly. This study investigated the anti-Human coronavirus NL63 (HCoV-NL63) activity of the methanol extract of S. cusia leaf and its major components. The methanol extract of S. cusia leaf effectively inhibited the cytopathic effect (CPE) and virus yield (IC(50) = 0.64 μg/mL) in HCoV-NL63-infected cells. Moreover, this extract potently inhibited the HCoV-NL63 infection in a concentration-dependent manner. Among the six components identified in the methanol extract of S. cusia leaf, tryptanthrin and indigodole B (5aR-ethyltryptanthrin) exhibited potent antiviral activity in reducing the CPE and progeny virus production. The IC(50) values against virus yield were 1.52 μM and 2.60 μM for tryptanthrin and indigodole B, respectively. Different modes of time-of-addition/removal assay indicated that tryptanthrin prevented the early and late stages of HCoV-NL63 replication, particularly by blocking viral RNA genome synthesis and papain-like protease 2 activity. Notably, tryptanthrin (IC(50) = 0.06 μM) and indigodole B (IC(50) = 2.09 μM) exhibited strong virucidal activity as well. This study identified tryptanthrin as the key active component of S. cusia leaf methanol extract that acted against HCoV-NL63 in a cell-type independent manner. The results specify that tryptanthrin possesses antiviral potential against HCoV-NL63 infection.
has issue date	2020-02-27(xsd:dateTime)
bibo:doi	10.3390/biom10030366
bibo:pmid	32120929
has license	cc-by
sha1sum (hex)	b72367f5f0a0866aec4159d0886cc8090421e964
schema:url	https://doi.org/10.3390/biom10030366
resource representing a document's title	Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63
has PubMed Central identifier	PMC7175275
has PubMed identifier	32120929
schema:publication	Biomolecules
resource representing a document's body	covid:b72367f5f0a0866aec4159d0886cc8090421e964#body_text
is schema:about of	named entity 'RNA' named entity 'production' named entity 'infection' named entity 'virucidal' named entity 'viral' named entity 'extract' covid:arg/b72367f5f0a0866aec4159d0886cc8090421e964 named entity 'late' named entity 'leaf' named entity 'antiviral' named entity 'potent' named entity 'infection' named entity 'genome synthesis' named entity 'yield' named entity 'leaf' named entity 'leaf' named entity 'activity' named entity 'independent' named entity 'methanol' named entity 'virus' named entity 'betulin' named entity 'immunocompromised' named entity 'HCoV-NL63' named entity 'Chinese herbal medicine' named entity 'HCoV-NL63' named entity 'cytopathic effect' named entity 'antiviral' named entity 'infection' named entity 'Nees' named entity 'infection' named entity 'antiviral' named entity 'Thermo Fisher' named entity 'HCoV-NL63' named entity 'reverse transcription polymerase chain reaction' named entity 'di-3' named entity 'supernatant' named entity 'oxidative stress' named entity 'virus' named entity 'virucidal' named entity 'reagent' named entity 'gene' named entity 'RNA' named entity 'HCoV-NL63' named entity 'viruses' named entity 'microscope' named entity 'epithelial' named entity 'PLP2' named entity 'HCoV-NL63' named entity 'multiplicity of infection' named entity 'virus' named entity 'recombinant' named entity 'cytotoxicity' named entity 'IgG antibodies' named entity 'virucidal' named entity 'chemical constituents' named entity 'HCoV-NL63' named entity 'antiviral activity' named entity 'HCoV-NL63' named entity 'tinctorial' named entity '0.01' named entity 'virions' named entity 'BamH1' named entity 'Taoyuan City' named entity 'antiviral' named entity 'One-way ANOVA' named entity 'dose-dependent' named entity 'methanol' named entity 'virucidal' named entity 'chromogen' named entity 'TLR4' named entity 'Novagen' named entity 'virus' named entity 'titer' named entity 'HCoV-NL63' named entity 'cell-type'

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