About: A C-terminal Pfs48/45 malaria transmission-blocking vaccine candidate produced in the baculovirus expression system

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About: A C-terminal Pfs48/45 malaria transmission-blocking vaccine candidate produced in the baculovirus expression system Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A C-terminal Pfs48/45 malaria transmission-blocking vaccine candidate produced in the baculovirus expression system
Creator	Miura, Kazutoyo Hickey, John Joshi, Sangeeta Lee, Shwu-Maan Plieskatt, Jordan Richter King, Volkin, David
Source	PMC
abstract	The Plasmodium falciparum gametocyte surface protein, Pfs48/45, is a potential target for malaria transmission-blocking vaccines. However, due to its size and complexity, expression of the full-length protein has been difficult, leading to focus on the C-terminal six cysteine domain (6C) with the use of fusion proteins to facilitate expression and folding. In this study, we utilized the baculovirus system to evaluate the expression of three Pfs48/45 proteins including the full-length protein, the 6C domain fragment and the 6C domain mutant to prevent glycosylation. Expression of the recombinant Pfs48/45 proteins was conducted in super Sf9 cells combined with the use of tunicamycin to prevent N-glycosylation. The proteins were then evaluated as immunogens in mice to demonstrate the induction of functionally active polyclonal antibody responses as measured in the standard membrane feeding assay (SMFA). Only the 6C protein was found to exhibit significant transmission-reducing activity. Further characterization of the biologically active 6C protein demonstrated it was homogeneous in terms of size, charge, conformation, absence of glycosylation, and containing proper disulfide bond pairings. This study presents an alternative expression system, without the need of a fusion protein partner, for the Pfs48/45 6C protein fragment including further evaluation as a potential transmission-blocking vaccine candidate.
has issue date	2020-01-15(xsd:dateTime)
bibo:doi	10.1038/s41598-019-57384-w
bibo:pmid	31942034
has license	cc-by
sha1sum (hex)	b6e1bfae64098d99e7e244f8115b406084dfa7d2
schema:url	https://doi.org/10.1038/s41598-019-57384-w
resource representing a document's title	A C-terminal Pfs48/45 malaria transmission-blocking vaccine candidate produced in the baculovirus expression system
has PubMed Central identifier	PMC6962329
has PubMed identifier	31942034
schema:publication	Sci Rep
resource representing a document's body	covid:b6e1bfae64098d99e7e244f8115b406084dfa7d2#body_text
is schema:about of	named entity 'size' named entity 'prevent' named entity 'functionally' named entity 'system' named entity 'gametocyte' named entity 'expression system' named entity 'membrane' named entity 'baculovirus' named entity 'expression system' named entity 'SUPER' named entity 'INDUCTION' named entity 'EXHIBIT' named entity 'PRESENTS' named entity 'SYSTEM' named entity 'TO PREVENT' named entity 'MICE' named entity 'MEMBRANE' named entity 'ACTIVE' named entity 'VACCINES' named entity 'TUNICAMYCIN' named entity 'PLASMODIUM FALCIPARUM GAMETOCYTE' named entity 'DIFFICULT' named entity 'proper' named entity 'expression' named entity 'tunicamycin' named entity 'pairings' named entity 'C-terminal' named entity 'proteins' named entity 'size' named entity 'fusion protein' named entity 'proteins' named entity 'protein' named entity 'biologically active' named entity 'assay' named entity 'immunogens' named entity 'vaccine' named entity 'baculovirus' named entity 'tunicamycin' named entity 'Sf9' named entity 'fusion proteins' named entity 'protein' named entity 'C-terminal' named entity 'malaria transmission' named entity 'sarkosyl' named entity 'Isoelectric focusing' named entity 'disulfide' named entity 'IEF' named entity 'multiple comparison' named entity 'immunogen' named entity 'antibiotic' named entity 'SDS-PAGE' named entity 'post-translational modifications' named entity 'chromatogram' named entity 'reduced form' named entity 'process optimization' named entity 'protein expression' named entity 'multiplicity of infection' named entity 'baculovirus' named entity 'isoleucine' named entity 'denaturation' named entity 'baculovirus' named entity 'mass spectrometric analysis' named entity 'protein' named entity 'thiol groups' named entity 'secretion' named entity 'P. falciparum' named entity 'amino acid residue' named entity 'LC-MS' named entity 'mice' named entity 'hydrophobic' named entity 'secretion' named entity 'tunicamycin' named entity 'anion exchange' named entity 'alkaline phosphatase' named entity 'IgG'

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