About: Validation of γ-radiation and ultraviolet as a new inactivators for foot and mouth disease virus in comparison with the traditional methods

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About: Validation of γ-radiation and ultraviolet as a new inactivators for foot and mouth disease virus in comparison with the traditional methods Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Validation of γ-radiation and ultraviolet as a new inactivators for foot and mouth disease virus in comparison with the traditional methods
Creator	El Din Mahdy, Safy El-Din, Gamal El-Sayed, Ehab Fakhry, Hiam Hassanin, Ismail Ibrahim, El-Sayed Mossad, Wael
Source	Medline; PMC
abstract	AIM: The present work deals with different methods for foot and mouth disease virus (FMDV) inactivation for serotypes O/pan Asia, A/Iran05, and SAT-2/2012 by heat, gamma radiation, and ultraviolet (UV) in comparison with the traditional methods and their effects on the antigenicity of viruses for production of inactivated vaccines. MATERIALS AND METHODS: FMDV types O/pan Asia, A/Iran05, and SAT-2/2012 were propagated in baby hamster kidney 21 (BHK21) and titrated then divided into five parts; the first part inactivated with heat, the second part inactivated with gamma radiation, the third part inactivated with UV light, the fourth part inactivated with binary ethylamine, and the last part inactivated with combination of binary ethylamine and formaldehyde (BEI+FA). Evaluate the method of inactivation via inoculation in BHK21, inoculation in suckling baby mice and complement fixation test then formulate vaccine using different methods of inactivation then applying the quality control tests to evaluate each formulated vaccine. RESULTS: The effect of heat, gamma radiation, and UV on the ability of replication of FMDV “O/pan Asia, A/Iran05, and SAT-2/2012” was determined through BHK cell line passage. Each of the 9 virus aliquots titer 10(8) TCID(50) (3 for each strain) were exposed to 37, 57, and 77°C for 15, 30, and 45 min. Similarly, another 15 aliquots (5 for each strain) contain 1 mm depth of the exposed samples in petri-dish was exposed to UV light (252.7 nm wavelength: One foot distance) for 15, 30, 45, 60, and 65 min. Different doses of gamma radiation (10, 20, 25, 30, 35, 40, 45, 50, 55, and 60 KGy) were applied in a dose rate 0.551 Gy/s for each strain and repeated 6 times for each dose. FMDV (O/pan Asia, A/Iran05, and SAT-2/2012) were inactivated when exposed to heat ≥57°C for 15 min. The UV inactivation of FMDV (O/pan Asia and SAT-2) was obtained within 60 min and 65 min for type A/Iran05. The ideal dose for inactivation of FMDV (O/pan Asia, A/Iran05, and SAT-2/2012) with gamma radiation were 55-60 and 45 kGy, respectively. Inactivation of FMDV with binary was 20, 24 and 16 hr for O/pan Asia, A/Iran05, and SAT-2/2012, respectively while inactivation by (BEI+FA) was determined after 18, 19 and 11 hr for O/pan-Asia, A/Iran 05, and SAT-2/2012, respectively. The antigenicity of control virus before inactivation was 1/32, it was not changed after inactivation in case of gamma radiation and (BEI+FA) and slightly decrease to 1/16 in case of binary and declined to 1/2, 1/4 in case of heat and UV inactivation, respectively. The immune response induced by inactivated FMD vaccines by gamma radiation and (BEI+FA) lasted to 9 months post-vaccination, while the binary only still up to 8 months post-vaccination but heat and UV-inactivated vaccines were not effective. CONCLUSION: Gamma radiation could be considered a good new inactivator inducing the same results of inactivated vaccine by binary with formaldehyde (BEI+FA).
has issue date	2015-09-19(xsd:dateTime)
bibo:doi	10.14202/vetworld.2015.1088-1098
bibo:pmid	27047204
has license	cc-by
sha1sum (hex)	b6b06d62a3af9c36824d3c674f4d43d6d78d9073
schema:url	https://doi.org/10.14202/vetworld.2015.1088-1098
resource representing a document's title	Validation of γ-radiation and ultraviolet as a new inactivators for foot and mouth disease virus in comparison with the traditional methods
has PubMed Central identifier	PMC4774778
has PubMed identifier	27047204
schema:publication	Vet World
resource representing a document's body	covid:b6b06d62a3af9c36824d3c674f4d43d6d78d9073#body_text
is schema:about of	named entity 'ultraviolet' covid:arg/b6b06d62a3af9c36824d3c674f4d43d6d78d9073 named entity 'foot' named entity 'virus' named entity 'traditional methods' named entity 'foot and mouth disease virus' named entity 'ultraviolet' named entity 'viruses' named entity 'antigen' named entity 'Pirbright' named entity 'inoculation' named entity 'formaldehyde' named entity '37°C' named entity 'virus' named entity 'milk production' named entity 'formaldehyde' named entity 'antigen' named entity 'inoculation' named entity 'inoculation' named entity 'SAT-2' named entity 'MPV' named entity 'gamma radiation' named entity 'virus' named entity 'antigen' named entity 'hemolysis' named entity 'UV irradiation' named entity 'UV irradiation' named entity 'virus' named entity 'gamma ray' named entity 'vaccine' named entity 'antigen' named entity 'cloven-hoofed' named entity '37°C' named entity 'veronal' named entity 'virus' named entity 'albumin' named entity 'formaldehyde' named entity 'BHK' named entity 'virus' named entity 'kGy' named entity 'titer' named entity 'gamma radiation' named entity 'SAT-2' named entity 'allergic responses' named entity '37°C' named entity 'serum' named entity 'heat treatment' named entity 'BHK' named entity 'SNT' named entity 'veronal' named entity 'antigen' named entity 'gamma irradiation' named entity 'irradiation' named entity 'albumin' named entity '37°C' named entity 'clone' named entity '37°C' named entity 'UV light' named entity 'veronal' named entity 'cell culture' named entity 'WMGE' named entity 'serially diluted' named entity 'veronal' named entity 'immune response' named entity 'sheep' named entity 'virus' named entity 'SAT-2' named entity 'inactivated vaccine' named entity 'BHK' named entity 'virus' named entity 'ultraviolet' named entity 'SNT' named entity 'formalin' named entity 'cell cultures' named entity 'substrate'

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