About: SARS-CoV-2 infects brain choroid plexus and disrupts the blood-CSF-barrier

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About: SARS-CoV-2 infects brain choroid plexus and disrupts the blood-CSF-barrier Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 infects brain choroid plexus and disrupts the blood-CSF-barrier
Creator	Paul, David Albecka, Anna James, Leo Lancaster, Madeline Carter, Andrew Kellner, Max Mallery, Donna Pellegrini, Laura
Source	BioRxiv
abstract	Coronavirus disease-19 (COVID-19), caused by the SARS-CoV-2 virus, leads primarily to respiratory symptoms that can be fatal, particularly in at risk individuals. However, neurological symptoms have also been observed in patients, including headache, seizures, stroke, and fatigue. The cause of these complications is not yet known, and whether they are due to a direct infection of neural cells, such as neurons and astrocytes, or through indirect effects on supportive brain cells, is unknown. Here, we use brain organoids to examine SARS-CoV-2 neurotropism. We examine expression of the key viral receptor ACE2 in single-cell RNA sequencing (scRNA-seq) revealing that only a subset of choroid plexus cells but not neurons or neural progenitors express this entry factor. We then challenge organoids with both SARS-CoV-2 spike protein pseudovirus and live virus to demonstrate high viral tropism for choroid plexus epithelial cells but not stromal cells, and little to no infection of neurons or glia. We find that infected cells of the choroid plexus are an apolipoprotein and ACE2 expressing subset of epithelial barrier cells. Finally, we show that infection with live SARS-CoV-2 leads to barrier breakdown of the choroid plexus. These findings suggest that neurological complications may result from effects on the choroid plexus, an important barrier that normally prevents entry of immune cells and cytokines into the cerebrospinal fluid (CSF) and brain.
has issue date	2020-08-21(xsd:dateTime)
bibo:doi	10.1101/2020.08.20.259937
has license	biorxiv
sha1sum (hex)	b5dde8b1ed94713ea59122ed8a0e702c2cc79a2e
schema:url	https://doi.org/10.1101/2020.08.20.259937
resource representing a document's title	SARS-CoV-2 infects brain choroid plexus and disrupts the blood-CSF-barrier
schema:publication	bioRxiv
resource representing a document's body	covid:b5dde8b1ed94713ea59122ed8a0e702c2cc79a2e#body_text
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