About: Molecular characterization of canine coronavirus strains circulating in Brazil

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About: Molecular characterization of canine coronavirus strains circulating in Brazil Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of canine coronavirus strains circulating in Brazil
Creator	Moutinho Costa, Erika De Cá Ssia Nasser, Rita De Oliveira Bottino, Fernanda Garcia, Cubel Xavier De Castro, Tatiana
Source	Elsevier; Medline; PMC
abstract	To characterize canine coronavirus (CCoV) circulating in diarrheic puppies in Brazil, 250 fecal samples collected between 2006 and 2012 were tested. By using RT-PCR to partially amplify the M gene, CCoV RNA was detected in 30 samples. Sequence analysis of the M protein grouped eight strains with CCoV-I and another 19 with CCoV-II prototypes. To genotype/subtype the CCoV strains and assess the occurrence of single or multiple CCoV infections, RT-PCR of the S gene was performed, and 25/30 CCoV-positive strains amplified with one or two primer pairs. For 17/25 samples, single infections were detected as follows: six CCoV-I, nine CCoV-IIa and two CCoV-IIb. Eight samples were positive for more than one genotype/subtype as follows: seven CCoV-I/IIa and one CCoV-I/IIb. Sequence analysis revealed that the CCoV-I and IIa strains shared high genetic similarity to each other and to the prototypes. The Brazilian strains of CCoV-IIb displayed an aminoacid insertion that was also described in CCoV-IIb-UCD-1 and TGEV strains. Among the 25 CCoV-positive puppies, five had a fatal outcome, all but one of which were cases of mixed infection. The current study is the first reported molecular characterization of CCoV-I, IIa and IIb strains in Brazil.
has issue date	2014-01-10(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2013.10.002
bibo:pmid	24216489
has license	no-cc
sha1sum (hex)	b56a7a05df207cfba372ea95b1c34fe47a3ee786
schema:url	https://doi.org/10.1016/j.vetmic.2013.10.002
resource representing a document's title	Molecular characterization of canine coronavirus strains circulating in Brazil
has PubMed Central identifier	PMC7117457
has PubMed identifier	24216489
schema:publication	Vet Microbiol
resource representing a document's body	covid:b56a7a05df207cfba372ea95b1c34fe47a3ee786#body_text
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