About: Indirect Enzyme-Linked Immunosorbent Assay based on the nucleocapsid protein of SARS-like coronaviruses

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About: Indirect Enzyme-Linked Immunosorbent Assay based on the nucleocapsid protein of SARS-like coronaviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Indirect Enzyme-Linked Immunosorbent Assay based on the nucleocapsid protein of SARS-like coronaviruses
Creator	Shi, Zheng-Li Zhou, Peng Li, Yan Zhang, Yun-Zhi Ke, Zhen-Hua Yuan, Jun-Fa Zhang, Hua-Jun
Source	PMC
abstract	The nucleocapsid protein (N) is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus (SL-CoV) has a high similarity with that of SARS-CoV. In this study, the SL-CoV N protein was expressed in Escherichia coli, purified and used as antigen. An Indirect Enzyme-Linked Immunosorbent Assay (indirect ELISA) was developed for detection of SARS- or SL-CoV infections in bat populations. The detection of 573 bat sera with this indirect ELISA demonstrated that SL-CoVs consistently circulate in Rhinilophus species, further supporting the proposal that bats are natural reservoirs of SL-CoVs. This method uses 1–2 μl of serum sample and can be used for preliminary screening of infections by SARS- or SL-CoV with a small amount of serum sample.
has issue date	2009-04-14(xsd:dateTime)
bibo:doi	10.1007/s12250-009-3037-0
has license	no-cc
sha1sum (hex)	b5042f7a9e88b4f64c47b297deaf0bc24f0be591
schema:url	https://doi.org/10.1007/s12250-009-3037-0
resource representing a document's title	Indirect Enzyme-Linked Immunosorbent Assay based on the nucleocapsid protein of SARS-like coronaviruses
has PubMed Central identifier	PMC7090808
schema:publication	Virol Sin
resource representing a document's body	covid:b5042f7a9e88b4f64c47b297deaf0bc24f0be591#body_text
is schema:about of	named entity 'PROTEIN ' named entity 'Escherichia coli' covid:arg/b5042f7a9e88b4f64c47b297deaf0bc24f0be591 named entity 'RESULTING IN' named entity 'BAT SARS-LIKE CORONAVIRUS' named entity 'RESERVOIRS' named entity 'SCREENING' named entity 'PROTEIN ' named entity 'INFECTIONS' named entity 'NATURAL' named entity 'ANTIGEN' named entity 'SERUM SAMPLE' named entity 'METHOD' named entity 'A MAJOR' named entity '1-2' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'EXPRESSED' named entity 'SARS-COV' named entity 'BAT' named entity 'STUDY' named entity 'CIRCULATE' named entity 'IS A' named entity 'PRELIMINARY' named entity 'DETECTION' named entity 'INDIRECT' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'PURIFIED' named entity 'INDIRECT' named entity 'BATS' named entity 'A SMALL AMOUNT' named entity 'NUCLEOCAPSID PROTEIN' named entity 'LIKE' named entity 'SARS' named entity 'SIMILARITY' named entity 'ELISA' named entity 'SERA' named entity '573' named entity 'CAUSATIVE AGENT' named entity 'CORONAVIRUSES' named entity 'BASED' named entity 'POPULATIONS' named entity 'SPECIES' named entity 'SUPPORTING' named entity 'PROPOSAL' named entity 'STRUCTURAL PROTEIN' named entity 'SARS' named entity 'VIRUS' named entity 'COVS' named entity 'N PROTEIN' named entity 'USED' named entity 'DEMONSTRATED' named entity 'USES' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'ESCHERICHIA COLI' named entity 'HIGH' named entity 'CORONAVIRUSES' named entity 'NUCLEOCAPSID PROTEIN' named entity 'protein' named entity 'circulate' named entity 'The N' named entity 'This' named entity 'bats' named entity 'demonstrated' named entity 'virus' named entity 'detection' named entity 'SARS' named entity 'causative agent' named entity 'protein' named entity 'antigen' named entity 'serum' named entity 'SARS-CoV' named entity 'Escherichia coli' named entity 'Nucleocapsid' named entity 'SARS-CoV' named entity 'serum' named entity 'kDa' named entity 'Escherichia coli'

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