About: Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA

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About: Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA
Creator	Liu, Qing Bian, Xiaoping Kong, Qingke Shen, Xuegang
source	PMC
abstract	BACKGROUND: Enterobacterial common antigen (ECA) is a family-specific surface antigen shared by all members of the Enterobacteriaceae family. Previous studies showed that the loss of ECA results in Salmonella attenuation, indicating its usefulness as a vaccine candidate for Salmonella infection, but no studies have shown whether the mutation resulting from the deletion of the ECA operon in conjunction with other mutations could be used as an antigen vehicle for heterologous protein antigen delivery. RESULTS: In this study, we introduced a nonpolar, defined ECA operon deletion into wild-type S. Typhimurium χ3761 and an attenuated vaccine strain χ9241, obtaining two isogenic ECA operon mutants, namely, χ12357 and χ12358, respectively. A number of in vitro and in vivo properties of the mutants were analyzed. We found that the loss of ECA did not affect the growth, lipopolysaccharide (LPS) production and motility of S. Typhimurium wild type strain χ3761 and its attenuated vaccine strain χ9241 but significantly affected the virulence when administered orally to BALB/c mice. Furthermore, the effects of the ECA mutation on the immunogenicity of a recombinant S. Typhimurium vaccine strain χ9241 when delivering the pneumococcal antigen PspA were determined. The result showed that the total anti-PspA IgG level of χ12358 (pYA4088) was slightly lower than that of χ9241 (pYA4088), but the protection rate was not compromised. CONCLUSIONS: ECA affects virulence and benefits the Th2 immunity of Salmonella Typhimurium, therefore, it is feasible to use a reversible ECA mutant mode to design future Salmonella vaccine strains for heterologous protective antigens.
has issue date	2020-06-08(xsd:dateTime)
bibo:doi	10.1186/s12866-020-01837-0
has license	cc-by
sha1sum (hex)	b4da44f816dc00c893e8ab4302e1f400115bd2ae
schema:url	https://doi.org/10.1186/s12866-020-01837-0
resource representing a document's title	Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA
has PubMed Central identifier	PMC7278252
schema:publication	BMC Microbiol
resource representing a document's body	covid:b4da44f816dc00c893e8ab4302e1f400115bd2ae#body_text
is schema:about of	named entity 'deletion' named entity 'mutants' named entity 'orally' named entity 'vaccine' named entity 'mice' named entity 'Streptococcus' named entity 'delivering' named entity 'TYPE STRAIN' named entity 'ATTENUATED VACCINE' named entity 'WILD-TYPE' named entity 'TOTAL' named entity 'ITS' named entity 'RECOMBINANT' named entity 'STRAINS' named entity 'IN VITRO' named entity 'PREVIOUS' named entity 'FUTURE' named entity 'candidate' named entity 'deletion' named entity 'loss' named entity 'antigen' named entity 'protein' named entity 'surface' named entity 'attenuated vaccine' named entity 'motility' named entity 'LPS' named entity 'introduced' named entity 'vaccine' named entity 'heterologous' named entity 'virulence' named entity 'isogenic' named entity 'administered orally' named entity 'Enterobacteriaceae' named entity 'heterologous' named entity 'Salmonella infection' named entity 'attenuated vaccine' named entity 'operon' named entity 'nonpolar' named entity 'vaccine' named entity 'live attenuated' named entity 'Streptococcus pneumoniae' named entity 'antigen' named entity 'virulence' named entity 'gene cluster' named entity 'vaccine' named entity 'immunogenicity' named entity 'antigen' named entity 'result' named entity 'wild type' named entity 'active ingredient' named entity 'patent' named entity 'wec' named entity 'recombinant' named entity 'operon' named entity '37°C' named entity '50% lethal dose' named entity 'yield strain' named entity 'vaccines' named entity 'IgG1' named entity 'murine' named entity 'inoculum' named entity 'E. coli' named entity 'IgG1' named entity 'BALB/c' named entity 'Experimental Animal' named entity 'Polystyrene' named entity 'gene cluster' named entity 'wec' named entity 'lysis buffer' named entity 'Klebsiella' named entity 'trisaccharide' named entity 'infection' named entity 'vaccine' named entity 'monoclonal antibody' named entity 'S. pneumoniae' named entity '37°C' named entity 'BALB/c' named entity 'S. Typhimurium' named entity 'bile salts' named entity 'peritonitis'

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