About: Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663
Creator	Maeda, Ken Morikawa, Shigeru Matsuura, Yoshiharu Terada, Yutaka Kamitani, Wataru Kuroda, Yudai
Source	Medline; PMC
abstract	Feline infectious peritonitis (FIP) is one of the most important infectious diseases in cats and is caused by feline coronavirus (FCoV). Tissue culture-adapted type I FCoV shows reduced FIP induction in experimental infections, which complicates the understanding of FIP pathogenesis caused by type I FCoV. We previously found that the type I FCoV strain C3663 efficiently induces FIP in specific-pathogen-free cats through the naturally infectious route. In this study, we employed a bacterial artificial chromosome-based reverse genetics system to gain more insights into FIP caused by the C3633 strain. We successfully generated recombinant virus (rC3663) from Fcwf-4 cells transfected with infectious cDNA that showed growth kinetics similar to those shown by the parental virus. Next, we constructed a reporter C3663 virus carrying the nanoluciferase (Nluc) gene to measure viral replication with high sensitivity. The inhibitory effects of different compounds against rC3663-Nluc could be measured within 24 h postinfection. Furthermore, we found that A72 cells derived from canine fibroblasts permitted FCoV replication without apparent cytopathic effects. Thus, our reporter virus is useful for uncovering the infectivity of type I FCoV in different cell lines, including canine-derived cells. Surprisingly, we uncovered aberrant viral RNA transcription of rC3663 in A72 cells. Overall, we succeeded in obtaining infectious cDNA clones derived from type I FCoV that retained its virulence. Our recombinant FCoVs are powerful tools for increasing our understanding of the viral life cycle and pathogenesis of FIP-inducing type I FCoV. IMPORTANCE Feline coronavirus (FCoV) is one of the most significant coronaviruses, because this virus induces feline infectious peritonitis (FIP), which is a lethal disease in cats. Tissue culture-adapted type I FCoV often loses pathogenicity, which complicates research on type I FCoV-induced feline infectious peritonitis (FIP). Since we previously found that type I FCoV strain C3663 efficiently induces FIP in specific-pathogen-free cats, we established a reverse genetics system for the C3663 strain to obtain recombinant viruses in the present study. By using a reporter C3663 virus, we were able to examine the inhibitory effect of 68 compounds on C3663 replication in Fcwf-4 cells and infectivity in a canine-derived cell line. Interestingly, one canine cell line, A72, permitted FCoV replication but with low efficiency and aberrant viral gene expression.
has issue date	2019-08-02(xsd:dateTime)
bibo:doi	10.1128/jvi.01208-19
bibo:pmid	31375588
has license	bronze-oa
sha1sum (hex)	b2e84eaaafdad0141daedef80cc2a242e3c27709
schema:url	https://doi.org/10.1128/jvi.01208-19
resource representing a document's title	Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663
has PubMed Central identifier	PMC6803248
has PubMed identifier	31375588
schema:publication	Journal of Virology
resource representing a document's body	covid:b2e84eaaafdad0141daedef80cc2a242e3c27709#body_text
is schema:about of	named entity 'USEFUL' named entity 'TISSUE CULTURE' named entity 'ABERRANT' named entity 'TO MEASURE' named entity 'Feline infectious peritonitis' named entity 'pathogenesis' named entity 'RNA transcription' named entity 'virus' named entity 'fibroblasts' named entity 'FIP' named entity 'infectivity' named entity 'virus' named entity 'FIP' named entity 'feline coronavirus' named entity 'protein' named entity 'PCR' named entity 'PCR' named entity 'real-time RT-PCR' named entity 'receptor' named entity 'IgG' named entity 'Basel' named entity 'recombination' named entity 'genetic marker' named entity 'lopinavir' named entity 'GST' named entity '0.01' named entity 'cell lines' named entity 'inoculate' named entity 'Tekes' named entity 'RT-PCR' named entity '3C-like protease' named entity 'phosphate-buffered saline' named entity 'primary antibodies' named entity '0.01' named entity 'virus' named entity 'RT-PCR' named entity 'horseradish peroxidase' named entity 'UTR' named entity 'ligation' named entity 'antibody-dependent enhancement of infection' named entity 'genetic marker' named entity 'human immunodeficiency virus type 1' named entity 'RT-PCR' named entity 'Lipofectamine' named entity 'cell line' named entity 'plasmid' named entity 'MDCK' named entity 'receptor' named entity 'gene' named entity 'Merck Millipore' named entity 'BALB/c' named entity 'Belgium' named entity 'cell lines' named entity 'Journal of Virology' named entity 'FBS' named entity 'titration' named entity 'Billerica' named entity 'adsorption' named entity 'lopinavir' named entity 'TMPRSS2' named entity 'virus' named entity 'Sanger sequencing' named entity 'AIDS treatment' named entity 'real-time PCR' named entity 'MAb' named entity 'genetic marker' named entity 'RT-PCR' named entity 'progeny viruses' named entity 'EcoRI' named entity 'ORF' named entity 'PCR' named entity 'genetic marker' named entity 'UTR' named entity 'BamHI' named entity 'MDCK' named entity 'Japan'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software