About: Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus

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About: Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus
Creator	Lei, Jing Zhou, Jiyong Yan, Yan Liao, Min Jin, Yulan Mo, Kaikun Shi, Tingting Sun, Dongnan
Source	Elsevier; Medline; PMC
abstract	Abstract Infectious bronchitis virus (IBV) continues to be one of the most important poultry pathogens worldwide. The current commercially available enzyme-linked immunosorbent assay (ELISA) kits for IBV specific antibody detection are mostly based on the whole virion, and few serological tests based on nonstructural proteins of IBV have been developed. Herein, an alternative indirect ELISA for detection of IBV antibody was developed with IBV nonstructural protein 5 (nsp5) produced by Escherichia coli. Using an indirect immunofluorescence assay (IFA) and a commercial ELISA kit as reference, we optimized the nsp5-ELISA and determined its cut-off as 0.12. The diagnostic sensitivity (DSN), specificity (DSP) and accuracy of the nsp5-ELISA were 93.11%, 95.38% and 93.33%, respectively, compared with IFA in 660 field serum samples, and were 98.11%, 95.00% and 97.62%, respectively, compared with the commercial IBV ELISA kit (IDEXX) in 126 field sera samples. Furthermore, a time course of IBV specific antibody level detected by nsp5-ELISA following IBV infection and vaccination is consistent with that of IBV antibody detected by the commercial ELISA kit. The results presented in this study indicate that nsp5-ELISA has the potential to serve as a rapid, reliable and cost-effective method for IBV antibody detection. This study is the first to report the development of an nsp-based ELISA to detect an antibody to IBV.
has issue date	2017-05-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2017.01.026
bibo:pmid	28223026
has license	els-covid
sha1sum (hex)	b2cd93fc69bc2a613d20be9bc9311a7ecf23005e
schema:url	https://doi.org/10.1016/j.jviromet.2017.01.026
resource representing a document's title	Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus
has PubMed Central identifier	PMC7113779
has PubMed identifier	28223026
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:b2cd93fc69bc2a613d20be9bc9311a7ecf23005e#body_text
is schema:about of	named entity 'detected' named entity 'developed' named entity 'antibody' named entity 'worldwide' named entity 'nonstructural protein' named entity 'cost-effective' named entity 'IBV' named entity 'antibody' covid:arg/b2cd93fc69bc2a613d20be9bc9311a7ecf23005e named entity 'DSN' named entity 'vaccination' named entity 'IBV' named entity 'presented' named entity 'kit' named entity 'Enzyme-linked immunosorbent assay' named entity 'based' named entity 'detection' named entity 'IBV' named entity 'serum' named entity 'continues' named entity 'IBV' named entity 'sensitivity' named entity 'antibody' named entity 'nsp' named entity 'vaccination' named entity 'IBV' named entity 'IBV' named entity 'immunofluorescence assay' named entity 'poultry' named entity 'serological tests' named entity 'IBV' named entity 'IBV' named entity 'IBV' named entity 'serum samples' named entity 'antiserum' named entity 'H52' named entity 'infection' named entity 'cut-off' named entity 'nsp5' named entity 'antibody' named entity 'cDNA' named entity 'H52' named entity 'DF-1' named entity 'distilled water' named entity '1:200' named entity 'IBV' named entity 'IBV' named entity 'Fermentas' named entity 'IBV' named entity 'antibody' named entity 'nsp5' named entity 'DF-1' named entity 'cell line' named entity 'nsp5' named entity 'RNA' named entity 'nsp5' named entity 'nsp5' named entity 'virulent' named entity 'SalI' named entity 'pH 9' named entity 'reference method' named entity 'nsp5' named entity 'E. coli' named entity 'antibody' named entity 'pathogens' named entity 'serotypes' named entity 'nsp5' named entity 'Mpro' named entity 'NDV' named entity 'IBV' named entity 'IBV' named entity 'antibodies' named entity 'antigen' named entity 'IBV'

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