About: Sequence requirements for RNA strand transfer during nidovirus discontinuous subgenomic RNA synthesis

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About: Sequence requirements for RNA strand transfer during nidovirus discontinuous subgenomic RNA synthesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sequence requirements for RNA strand transfer during nidovirus discontinuous subgenomic RNA synthesis
Creator	Spaan, Willy Snijder, Eric Pasternak, Alexander Van Den Born, Erwin
Source	PMC
abstract	Nidovirus subgenomic mRNAs contain a leader sequence derived from the 5′ end of the genome fused to different sequences (‘bodies’) derived from the 3′ end. Their generation involves a unique mechanism of discontinuous subgenomic RNA synthesis that resembles copy-choice RNA recombination. During this process, the nascent RNA strand is transferred from one site in the template to another, during either plus or minus strand synthesis, to yield subgenomic RNA molecules. Central to this process are transcription-regulating sequences (TRSs), which are present at both template sites and ensure the fidelity of strand transfer. Here we present results of a comprehensive co-variation mutagenesis study of equine arteritis virus TRSs, demonstrating that discontinuous RNA synthesis depends not only on base pairing between sense leader TRS and antisense body TRS, but also on the primary sequence of the body TRS. While the leader TRS merely plays a targeting role for strand transfer, the body TRS fulfils multiple functions. The sequences of mRNA leader–body junctions of TRS mutants strongly suggested that the discontinuous step occurs during minus strand synthesis.
has issue date	2001-12-17(xsd:dateTime)
bibo:doi	10.1093/emboj/20.24.7220
bibo:pmid	11742998
has license	green-oa
sha1sum (hex)	b2897e1277f56641193a6db73825f707eed3e4c9
schema:url	https://doi.org/10.1093/emboj/20.24.7220
resource representing a document's title	Sequence requirements for RNA strand transfer during nidovirus discontinuous subgenomic RNA synthesis
has PubMed Central identifier	PMC125340
has PubMed identifier	11742998
schema:publication	The EMBO Journal
resource representing a document's body	covid:b2897e1277f56641193a6db73825f707eed3e4c9#body_text
is schema:about of	named entity 'TRS' named entity 'body' named entity 'TRS' named entity 'TRS' named entity 'RNA' named entity 'leader sequence' named entity 'bodies' named entity 'GENERATION' named entity 'RNA' named entity 'BODY' named entity 'SYNTHESIS' named entity 'COMPREHENSIVE' named entity 'MUTANTS' named entity 'MOLECULES' named entity 'STRAND' named entity 'RNA RECOMBINATION' named entity 'RNA SYNTHESIS' named entity 'BASE PAIRING' named entity 'TEMPLATE' named entity 'SENSE' named entity 'DISCONTINUOUS' named entity 'MUTAGENESIS' named entity 'TRS' named entity 'TRANSFER' named entity 'SITE' named entity 'EQUINE ARTERITIS VIRUS' named entity 'TRANSCRIPTION' named entity 'END' named entity 'BUT' named entity 'MULTIPLE' named entity 'PROCESS' named entity 'UNIQUE' named entity 'TARGETING ROLE' named entity 'CONTAIN' named entity 'HERE' named entity 'EITHER' named entity 'FUSED TO' named entity 'DIFFERENT' named entity 'REGULATING' named entity 'SEQUENCE' named entity 'ANTISENSE' named entity 'DERIVED' named entity 'ENSURE' named entity 'THEIR' named entity 'SEQUENCES' named entity 'VARIATION' named entity 'STEP' named entity 'PRIMARY' named entity 'CENTRAL' named entity 'MRNA' named entity 'YIELD' named entity 'MRNAS' named entity 'TRANSFERRED FROM' named entity 'OCCURS DURING' named entity 'FUL' named entity 'FUNCTIONS' named entity 'GENOME' named entity 'COPY' named entity 'MECHANISM' named entity 'RESULTS' named entity 'PRESENT' named entity 'LEADER SEQUENCE' named entity 'CHOICE' named entity 'PLAYS' named entity 'STUDY' covid:arg/b2897e1277f56641193a6db73825f707eed3e4c9 named entity 'equine arteritis virus' named entity 'strand' named entity 'body' named entity 'TRS' named entity 'discontinuous' named entity 'step' named entity 'discontinuous' named entity 'TRS' named entity 'transcription' named entity 'leader sequence' named entity 'recombination' named entity 'Nidovirus'

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