About: Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections

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About: Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections
Creator	Ma, Xue-Jun Wang, Hao Wang, Le Yang, Shuo Zhang, Dan Feng, Zhi-Shan Li, Gui-Xia Zhao, Meng-Chuan Zhou, Hang-Yu
source	Elsevier; Medline; PMC
abstract	Abstract Respiratory Pathogen 13 Detection Kit (13× kit) is able to simultaneously detect 11 respiratory viruses, Mycoplasma pneumoniae (MP) and Chlamydia in a single reaction. Using 572 Nasopharyngeal aspirates collected from hospitalized children, the clinical performance of 13× kit for detecting 11 respiratory viruses was evaluated in comparison with a routinely used 2-tube multiplex reverse transcription PCR assay (2-tube assay) at provincial Centers for Disease Control and Prevention in China. The clinical performance of 13× kit for detecting MP and Chlamydia was evaluated by commercial real-time quantitative PCR (qPCR) kits or sequencing. For tested viruses, the assay concordance was 95.98% and the kappa coefficient was 0.89. All the MP and Chlamydia positive samples detected by 13× kit were confirmed as true positives. The utilization of the 13× kit in clinical settings will be helpful for doctors to assess clinical outcome according to virus type or multiple infections, and to limit the use of antibiotics.
has issue date	2017-06-30(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2017.03.010
bibo:pmid	28365060
has license	els-covid
sha1sum (hex)	b276e4646eab1d824d4a4a1955507415bbdd63c5
schema:url	https://doi.org/10.1016/j.diagmicrobio.2017.03.010
resource representing a document's title	Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections
has PubMed Central identifier	PMC7132751
has PubMed identifier	28365060
schema:publication	Diagnostic Microbiology and Infectious Disease
resource representing a document's body	covid:b276e4646eab1d824d4a4a1955507415bbdd63c5#body_text
is schema:about of	named entity 'Disease Control' named entity 'collected' named entity 'limit' named entity 'CHLAMYDIA' named entity 'ASSAY' named entity 'CONFIRMED' named entity 'CHLAMYDIA' named entity 'HELPFUL' named entity 'DOCTORS' named entity '282' named entity 'TRUE' named entity 'MULTIPLEX' named entity 'COMMERCIAL' named entity 'COLLECTED' named entity 'UTILIZATION' named entity 'EVALUATED' named entity 'NASOPHARYNGEAL' named entity 'CENTERS FOR DISEASE CONTROL AND PREVENTION' named entity 'DETECTED' named entity 'CHINA' named entity 'PCR ASSAY' named entity 'SIMULTANEOUSLY' named entity 'DETECTION' named entity 'USED' named entity 'DETECTING' named entity 'REAL-TIME QUANTITATIVE PCR' named entity 'CONCORDANCE' named entity 'TUBE' named entity 'ASPIRATES' named entity 'SINGLE' named entity 'TUBE' named entity 'DETECT' named entity 'COEFFICIENT' named entity 'MULTIPLE INFECTIONS' named entity 'SEQUENCING' named entity 'USING' named entity 'TESTED' named entity 'ASSESS' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'HOSPITALIZED CHILDREN' named entity 'RESPIRATORY VIRUSES' covid:arg/b276e4646eab1d824d4a4a1955507415bbdd63c5 named entity 'MULTIPLEX' named entity 'RESPIRATORY VIRUSES' named entity 'COMPARISON' named entity 'USE OF' named entity 'POSITIVE' named entity 'CLINICAL SETTINGS' named entity 'HOSPITALIZED CHILDREN' named entity 'CLINICAL EVALUATION' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'PCR ASSAY' named entity 'SINGLE' named entity 'REACTION' named entity 'LIMIT' named entity 'CLINICAL' named entity 'PERFORMANCE' named entity 'KAPPA' named entity 'RESPIRATORY' named entity 'PATHOGEN' named entity 'ANTIBIOTICS' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'SAMPLES' named entity 'VIRUSES' named entity 'VIRUS TYPE' named entity 'KIT' named entity 'DETECTION' named entity 'ACUTE RESPIRATORY INFECTIONS' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'NEW' named entity 'SIMULTANEOUS' named entity 'OUTCOME' named entity 'detected'

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