About: Evaluation of COVID-19 RT-qPCR test in multi-sample pools

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of COVID-19 RT-qPCR test in multi-sample pools
Creator	Aharony, Noga Argoetti, Amir Berenbaum, Dina Geffen, Yuval Halberthal, Michael Hashimshony, Tamar Kishony, Kuzli, Areen Mandel-Gutfreund, Yael Messer, Esther Shafran, Einat Szwarcwort-Cohen, Moran Tamar, Shaer Yelin, Idan Gandali, Nagham Kishony, Marilyn Shkedi, Omer Taub, Henry
Source	Medline; PMC
abstract	BACKGROUND: The recent emergence of SARS-CoV-2 lead to a current pandemic of unprecedented scale. Though diagnostic tests are fundamental to the ability to detect and respond, overwhelmed healthcare systems are already experiencing shortages of reagents associated with this test, calling for a lean immediately-applicable protocol. METHODS: RNA extracts of positive samples were tested for the presence of SARS-CoV-2 using RT-qPCR, alone or in pools of different sizes (2-, 4-, 8- ,16-, 32- and 64-sample pools) with negative samples. Transport media of additional 3 positive samples were also tested when mixed with transport media of negative samples in pools of 8. RESULTS: A single positive sample can be detected in pools of up to 32 samples, using the standard kits and protocols, with an estimated false negative rate of 10%. Detection of positive samples diluted in even up to 64 samples may also be attainable, though may require additional amplification cycles. Single positive samples can be detected when pooling either after or prior to RNA extraction. CONCLUSIONS: As it uses the standard protocols, reagents and equipment, this pooling method can be applied immediately in current clinical testing laboratories. We hope that such implementation of a pool test for COVID-19 would allow expanding current screening capacities thereby enabling the expansion of detection in the community, as well as in close organic groups, such as hospital departments, army units, or factory shifts.
has issue date	2020-05-02(xsd:dateTime)
bibo:doi	10.1093/cid/ciaa531
bibo:pmid	32358960
has license	no-cc
sha1sum (hex)	b1dc6c975b290058faee40892e00981f606d2e57
schema:url	https://doi.org/10.1093/cid/ciaa531
resource representing a document's title	Evaluation of COVID-19 RT-qPCR test in multi-sample pools
has PubMed Central identifier	PMC7197588
has PubMed identifier	32358960
schema:publication	Clin Infect Dis
resource representing a document's body	covid:b1dc6c975b290058faee40892e00981f606d2e57#body_text
is schema:about of	named entity 'Israel Institute of Technology' named entity 'Technion' named entity 'PCR' named entity 'PCR' named entity 'SARS-CoV-2' named entity 'infection' named entity 'RNA' named entity 'RNA' named entity 'virus' named entity 'epidemics' named entity 'detection limit' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'COVID' named entity 'Virology' named entity 'microliter' named entity 'RT-qPCR' named entity 'SARS-CoV-2' named entity 'Rambam Health Care Campus' named entity 'fluorescence' named entity 'COVID-19 tests' named entity 'Haifa' named entity 'RT-qPCR' named entity 'lysis buffer' named entity 'severe acute respiratory syndrome' named entity 'SARS-CoV-2' named entity 'WHO' named entity 'RT-qPCR' named entity 'assay' named entity 'RNA' named entity 'false-negative' named entity 'RNA extraction' named entity 'pathogen' named entity 'tests positive' named entity 'assay' named entity 'fever' named entity 'short supply' named entity 'quarantine' named entity 'epidemic' named entity 'deidentified' named entity 'infectious diseases' named entity 'RNA' named entity 'proof-of-concept' named entity 'false negative rate' named entity 'quarantine' named entity 'RNA extraction' named entity 'fluorescence' named entity 'healthcare providers' named entity 'RNA' named entity 'CDC' named entity 'diagnostic tests' named entity 'clinical samples' named entity 'TaqMan' named entity 'microliter' named entity 'healthcare services' named entity 'World Health Organization' named entity 'Rambam Health Care Campus' named entity 'RdRp' named entity 'fluorescence' named entity 'RNA' named entity 'viral load' named entity 'March 17' named entity 'virus' named entity 'potentially infectious'

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