About: A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder
Creator	Sheng, Xiuzhen Tang, Xiaoqian Xing, Jing Xu, Hongsen Zhan, Wenbin Abbady, Abdul Dalmo, Roy
source	PMC
abstract	Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM(+), CD4-1(+), CD4-2(+), CD8β(+) lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1(+), CD4-2(+), and CD8β(+) T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM(+) lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.
has issue date	2019-03-19(xsd:dateTime)
bibo:doi	10.3389/fimmu.2019.00499
bibo:pmid	30941134
has license	cc-by
sha1sum (hex)	b1cb9713cb3f669a5f4b63e04395fb4669312d5b
schema:url	https://doi.org/10.3389/fimmu.2019.00499
resource representing a document's title	A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder
has PubMed Central identifier	PMC6435001
has PubMed identifier	30941134
schema:publication	Front Immunol
resource representing a document's body	covid:b1cb9713cb3f669a5f4b63e04395fb4669312d5b#body_text
is schema:about of	named entity 'intramuscularly' named entity 'induction' named entity 'bacterial' named entity 'lymphocytes' named entity 'slight' named entity 'T lymphocytes' named entity 'immune' named entity 'peak' named entity 'response' named entity 'protein' named entity 'reached' named entity 'CD4' named entity 'pathology' named entity 'CD4' named entity 'liver' named entity 'Response' covid:arg/b1cb9713cb3f669a5f4b63e04395fb4669312d5b named entity 'DNA vaccine' named entity 'fish' named entity 'flounder' named entity 'kidney' named entity 'effects' named entity 'result' named entity 'plasmid' named entity 'antibodies' named entity 'efficacy' named entity 'protein' named entity 'compared' named entity 'fish' named entity 'infection' named entity 'immunization' named entity 'inoculated' named entity 'spleen' named entity 'vaccination' named entity 'Histopathological' named entity 'liver' named entity 'recombinant DNA' named entity 'vector' named entity 'protein' named entity 'CD4' named entity 'DNA vaccine' named entity 'Vibrio anguillarum' named entity 'vivo' named entity 'gene' named entity 'transcription' named entity 'RT-PCR' named entity 'Lymphocytes' named entity 'disease prevention' named entity 'forward scatter' named entity 'intramuscular' named entity 'CD4' named entity 'DNA vaccine' named entity 'plasmid' named entity 'Log-rank test' named entity 'laboratory conditions' named entity 'flounder' named entity 'ethanol' named entity 'DNA vaccine' named entity 'transfected' named entity 'rpoS' named entity 'vaccine efficacy' named entity 'antibodies' named entity 'transfected' named entity 'T-cell' named entity 'sodium dodecyl sulfate' named entity 'polyclonal antibody' named entity 'opsonizing' named entity 'protein' named entity 'liver' named entity 'immunogenic' named entity 'primary antibody' named entity 'vaccine' named entity 'DNA vaccines' named entity 'qPCR' named entity 'antibodies'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software