About: Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria

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About: Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria
Creator	Ghram, Abdeljelil Ducatez, Mariette Lachheb, Jihene Alloui, Nadir Barberis, Abdelheq Boudaoud, Amine Gorrill, Angelina Loupias, Josianne
Source	PMC
abstract	BACKGROUND: H9N2 avian influenza viruses (AIV) has a worldwide geographic distribution and affects poultry of different types of production. H9N2 AIV was first reported in the Northeast of Algeria in April 2017, following an outbreak associated with high mortality, in broiler flocks. In the present study, we report full-length genome sequences of AIV H9N2, and the detailed phylogeny and molecular genetic analyses. METHODS: Ten AIV H9N2 strains, collected in broiler flocks, were amplified in 9-day-old embryonated specific pathogen free (SPF) chicken eggs. Their full-length genomes were successfully sequenced and phylogenetic and molecular characterizations were conducted. RESULTS: Phylogenetic analysis showed that the isolates were monophyletic, grouped within the G-1 lineage and were very close to Moroccan and Algerian strains identified in 2016 and 2017, respectively. The low pathogenicity of the strains was confirmed by the sequence motif (335RSSR/GLF341) at the hemagglutinin (HA) cleavage site. An exclusive substitution (T197A) that had not been previously reported for H9N2 viruses; but, conserved in some pandemic H1N1 viruses, was observed. When compared to the G1-like H9N2 prototype, the studied strains showed one less glycosylation site in HA, but 2–3 additional ones in the stalk of the neuraminidase (NA). The HA protein harbored the substitution 234 L, suggesting binding preference to human-like receptors. The NA protein harbored S372A and R403W substitutions, previously detected in H9N2 from Asia and the Middle East, and especially in H2N2 and H3N2 strains that caused human pandemics. Different molecular markers associated with virulence and mammalian infections have been detected in the viral internal proteins. The matrix M2 protein possessed the S31N substitution associated with drug resistance. The non-structural 1 (NS1) protein showed the “GSEV” PDZ ligand (PL) C-terminal motif and no 80–84 deletion. CONCLUSION: Characterized Algerian AIV isolates showed mutations that suggest increased zoonotic potential. Additional studies in animal models are required to investigate the pathogenicity of these H9N2 AIV strains. Monitoring their evolution in both migratory and domestic birds is crucial to prevent transmission to humans. Implementation of adequate biosecurity measures that limit the introduction and the propagation of AIV H9N2 in Algerian poultry farm is crucial.
has issue date	2020-07-17(xsd:dateTime)
bibo:doi	10.1186/s12985-020-01377-z
bibo:pmid	32680533
has license	cc-by
sha1sum (hex)	b1a611cc036cd57b42300843f9a275fd46a86dd6
schema:url	https://doi.org/10.1186/s12985-020-01377-z
resource representing a document's title	Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria
has PubMed Central identifier	PMC7366561
has PubMed identifier	32680533
schema:publication	Virol J
resource representing a document's body	covid:b1a611cc036cd57b42300843f9a275fd46a86dd6#body_text
is schema:about of	named entity 'strains' named entity '2016' named entity 'sequenced' named entity 'Northeast' named entity 'flocks' named entity 'types' named entity 'motif' named entity 'avian influenza' named entity 'HA PROTEIN' named entity 'MOTIF' named entity 'SITE' named entity 'REPORTED' named entity 'PRESENT' named entity 'DIFFERENT' named entity 'EGGS' named entity 'NEURAMINIDASE' named entity 'LIKE' named entity 'PREFERENCE' named entity 'APRIL' named entity 'sequences' named entity '2017' named entity 'worldwide' named entity 'protein' named entity 'strains' named entity 'genome' named entity 'glycosylation' named entity 'viruses' named entity 'strains' named entity 'Moroccan' named entity 'production' named entity 'grouped' named entity 'analyses' named entity 'substitution' named entity 'broiler' named entity 'H9N2' named entity 'drug resistance' named entity 'H9N2' named entity 'viruses' named entity 'molecular markers' named entity 'genomes' named entity 'AIV' named entity 'monophyletic' named entity 'specific pathogen free' named entity 'poultry' named entity 'H9N2' named entity 'AIV' named entity 'cleavage site' named entity 'glycosylation' named entity 'H9N2' named entity 'H9N2' named entity 'nucleotide' named entity 'AIV' named entity 'ClustalW' named entity 'AIV' named entity 'HA protein' named entity 'Phylogenetic analyses' named entity 'India' named entity 'antivirals' named entity 'nucleoproteins' named entity 'sialic acids' named entity 'allantoic' named entity 'avian influenza viruses' named entity 'dibasic' named entity 'H9N2' named entity 'host specificity' named entity 'Chicken' named entity 'polymerase' named entity 'host range' named entity 'virulence' named entity 'HA protein' named entity 'gene' named entity 'Hong Kong' named entity 'allantoic' named entity 'M2 protein'

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