About: Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18

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About: Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18
Creator	Li, Xunliang Ren, Xiaofeng Ren, Yudong Suo, Siqingaowa Wang, Chao Li, Guangxing Meng, Fandan Li, Pengchong Su, Dingding Zarlenga, Dante
Source	Elsevier; Medline; PMC
abstract	Abstract Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea, a highly contagious enteric disease of swine. The Spike (S) protein is one of the main structural proteins of PEDV capable of inducing neutralizing antibodies in vivo. Herein, we generated three distinct DNA constructs in the eukaryotic expression plasmid pVAX1; one encoding the S protein [pVAX1-(PEDV-S)], the second encoding the N-terminal fragment (S1) [pVAX1-(PEDV-S1)] containing potent antigenic sites, and the third expressing the porcine interleukin-18 (pIL-18) [pVAX1-(IL-18)]. Immunofluorescence assays in BHK-21 cells demonstrated successful protein expression from all 3 constructs. Kunming mice were injected separately with each of these constructs or with a pVAX1-(PEDV-S1)/pVAX1-(IL-18) combination, an attenuated PEDV vaccine, or vector only control. Animals were examined for T lymphocyte proliferation, anti-PEDV antibodies, IFN-γ and IL-4 protein levels, and cytotoxic T cell function in mouse peripheral blood and spleen. In all cases, results showed that pVAX1-(PEDV-S) and the combination of pVAX1-(PEDV-S1) with pVAX1-(IL-18) induced the strongest responses; however, pIL-18 had no adjuvant effects when given in combination with pVAX1-(PEDV-S1).
has issue date	2012-08-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2012.05.007
bibo:pmid	22643071
has license	els-covid
sha1sum (hex)	b16a8d52deaefdf9cb5990c803c210cda56a7680
schema:url	https://doi.org/10.1016/j.virusres.2012.05.007
resource representing a document's title	Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18
has PubMed Central identifier	PMC7114480
has PubMed identifier	22643071
schema:publication	Virus Research
resource representing a document's body	covid:b16a8d52deaefdf9cb5990c803c210cda56a7680#body_text
is schema:about of	named entity 'combination' named entity 'attenuated' named entity 'Animals' named entity 'Immune' named entity 'PEDV' named entity 'SPIKE' named entity 'RESULTS' named entity 'IL-4' named entity 'RESPONSES' named entity 'CAUSATIVE AGENT' named entity '28S' named entity 'PROTEIN EXPRESSION' named entity 'INDUCED' named entity 'CONTAINING' named entity 'ADJUVANT' named entity 'STRUCTURAL PROTEINS' named entity 'SUCCESSFUL' named entity 'IFN' covid:arg/b16a8d52deaefdf9cb5990c803c210cda56a7680 named entity 'CELLS' named entity 'KUNMING' named entity 'CYTOTOXIC T CELL' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'DISTINCT' named entity 'NEUTRALIZING ANTIBODIES' named entity 'COMBINATION' named entity 'PORCINE' named entity 'GIVEN' named entity 'DISEASE' named entity 'MAIN' named entity 'PERIPHERAL BLOOD' named entity 'EXPRESSION' named entity 'MOUSE' named entity 'CASES' named entity 'HIGHLY' named entity 'ANIMALS' named entity 'INTERLEUKIN-18' named entity '90%' named entity 'PIL' named entity 'S PROTEIN' named entity 'MICE' named entity 'PROTEIN ' named entity 'DNA' named entity 'ANTIBODIES' named entity 'THESE' named entity 'EXAMINED FOR' named entity 'N-TERMINAL FRAGMENT' named entity 'SECOND' named entity 'INDUCING' named entity 'PROTEIN LEVELS' named entity 'SITES' named entity 'ATTENUATED' named entity 'IMMUNE RESPONSES' named entity 'T CELL FUNCTION' named entity 'SPIKE' named entity 'SWINE' named entity 'EUKARYOTIC' named entity 'BHK-21' named entity 'DEMONSTRATED' named entity 'PLASMID' named entity 'GENERATED' named entity 'ENCODING' named entity 'IN VIVO' named entity 'ENTERIC' named entity 'CAPABLE' named entity 'VECTOR' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'SPLEEN' named entity 'T LYMPHOCYTE PROLIFERATION' named entity 'ONE OF' named entity 'VACCINE' named entity 'DNA VACCINES'

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