About: Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assays

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assays Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assays
Creator	Casas, I Aguilar, J Coiras, M García, M Pé Rez-Breñ, P
Source	Medline; PMC
abstract	There is a need for rapid, sensitive, and accurate diagnosis of lower respiratory tract infections in children, elderly, and immunocompromised patients, who are susceptible to serious complications. The multiplex RT‐nested PCR assay has been used widely for simultaneous detection of non‐related viruses involved in infectious diseases because of its high specificity and sensitivity. A new multiplex RT‐PCR assay is described in this report. This approach includes nested primer sets targeted to conserve regions of human parainfluenza virus haemagglutinin, human coronavirus spike protein, and human enterovirus and rhinovirus polyprotein genes. It permits rapid, sensitive, and simultaneous detection and typing of the four types of parainfluenza viruses (1, 2, 3, 4AB), human coronavirus 229E and OC43, and the generic detection of enteroviruses and rhinoviruses. The testing of 201 clinical specimens with this multiplex assay along with other one formerly described by our group to simultaneously detect and type the influenza viruses, respiratory syncytial viruses, and a generic detection of all serotypes of adenovirus, covers the detection of most viruses causing respiratory infectious disease in humans. The results obtained were compared with conventional viral culture, immunofluorescence assay, and a third multiplex RT‐PCR assay for all human parainfluenza viruses types described previously. In conclusion, both multiplex RT‐PCR assays provide a system capable of detecting and identifying simultaneously 14 different respiratory viruses in clinical specimens with high sensitivity and specificity, being useful for routine diagnosis and survey of these viruses within the population. J. Med. Virol. 72:484–495, 2004. © 2004 Wiley‐Liss, Inc.
has issue date	2004-01-09(xsd:dateTime)
bibo:doi	10.1002/jmv.20008
bibo:pmid	14748074
has license	no-cc
sha1sum (hex)	b0d99caf199c4d1761b911f59037b2abe6af1457
schema:url	https://doi.org/10.1002/jmv.20008
resource representing a document's title	Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assays
has PubMed Central identifier	PMC7166637
has PubMed identifier	14748074
schema:publication	J Med Virol
resource representing a document's body	covid:b0d99caf199c4d1761b911f59037b2abe6af1457#body_text
is schema:about of	named entity 'Reverse' named entity 'Simultaneous' named entity 'coronaviruses' named entity 'RT-PCR' named entity 'adenovirus' named entity 'Paisley' named entity 'cell cultures' named entity 'viruses' named entity 'gene' named entity 'pathogens' named entity 'antibiotics' named entity 'cDNA' named entity 'RSV' named entity 'respiratory diseases' named entity 'RT-PCR' named entity 'primers' named entity 'human parainfluenza viruses' named entity 'Temecula' named entity 'amplification efficiency' named entity 'coronavirus' named entity 'cell lines' named entity 'type 1' named entity 'parainfluenza virus' named entity 'bronchiolitis' named entity 'parainfluenza virus' named entity 'viruses' named entity 'streptomycin' named entity 'pathogens' named entity 'enteroviruses' named entity 'RSV' named entity 'epidemiological studies' named entity 'picornavirus' named entity 'centrifugation' named entity 'clinical presentation' named entity 'penicillin' named entity 'respiratory illnesses' named entity '21.4%' named entity 'PCR primers' named entity 'Madrid' named entity 'coronaviruses' named entity 'influenza' named entity 'NCR' named entity 'multiplex assay' named entity 'nested PCR' named entity 'viral culture' named entity 'OC43' named entity 'melting temperatures' named entity 'cloned' named entity 'influenza A and B viruses' named entity 'viral culture' named entity 'primers' named entity 'wild type' named entity 'viruses' named entity 'multiplex assay' named entity 'elderly people' named entity 'cDNA' named entity 'enteroviruses' named entity 'multiplex assay' named entity 'influenza-like illness' named entity 'nested PCR' named entity 'viruses' named entity 'nested PCR' named entity 'diagnostic tools' named entity 'parainfluenza' named entity 'penicillin' named entity 'Chemicon' named entity 'respiratory disease' named entity 'coronaviruses' named entity 'virulent' named entity 'internal control' named entity 'viruses' named entity 'virus' named entity 'oligonucleotide primers' named entity 'echovirus' named entity 'Manassas' named entity 'aspirates' named entity 'epidemiological studies'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software