About: Analysis of the mechanism by which BALB/c mice having prior immunization with nucleocapsid protein of SARS-CoV develop severe pneumonia after SARS-CoV infection

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About: Analysis of the mechanism by which BALB/c mice having prior immunization with nucleocapsid protein of SARS-CoV develop severe pneumonia after SARS-CoV infection Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analysis of the mechanism by which BALB/c mice having prior immunization with nucleocapsid protein of SARS-CoV develop severe pneumonia after SARS-CoV infection
Creator	Kohara, Michinori Mizuno, Kyosuke Yasui, Fumihiko Kai, Chieko Yoneda, Misako Inoue, Shingo Morita, Kouichi Saito, Kousuke
Source	Elsevier; Medline; PMC
abstract	Abstract The precise mechanism of severe acute respiratory syndrome (SARS), which is caused by SARS-associated coronavirus (SARS-CoV), is still unclear. We generated recombinant vaccinia virus (rVV) LC16m8 strain which simultaneously expresses four structural proteins of SARS-CoV, including nucleocapsid (N), membrane (M), envelop (E), spike (S) proteins (rVV-NMES) and reported that old BALB/c mice having prior immunization with rVV-NMES develop severe pneumonia similar to those of control mice though rVV-NMES-immunized mice showed lower pulmonary viral titer than in the control mice. Furthermore, we determined which SARS-CoV structural protein for the prior rVV-immunization is responsible for the severe pneumonia after the SARS-CoV infection as observed in the rVV-NMES-immunized mice. Old BALB/c mice were inoculated intradermally with rVV that expressed each structural proteins of SARS-CoV (rVV-N, -M, -E, or -S) with or without rVV-S and then infected intranasally with SARS-CoV more than 4 weeks later. At 9 days after SARS-CoV infection, the rVV-N-immunized mice show more severe pneumonia than in other groups. Furthermore, significant up-regulation of Th1 (IL-2)- and Th2 (IL-4 and IL-5)-bias cytokines and down-regulation of anti-inflammatory cytokine (IL-10 and TGF-β) were observed in rVV-N-immunized mice, resulting in the intensive infiltration of immunocompetent cells into the lung. In contrast, rVV-S-immunized mice showed only low pulmonary viral tier and slight pneumonia. However, the mice having co-immunization with both rVV-N and rVV-S showed severe pneumonia though their pulmonary viral titer was low. These results suggest that an excessive host immune response against the N protein of SARS-CoV is involved in severe pneumonia caused by SARS-CoV infection. These findings increase our understanding of the pathogenesis of SARS.
has issue date	2010-12-31(xsd:dateTime)
bibo:doi	10.1016/j.provac.2010.03.009
bibo:pmid	32288911
has license	els-covid
sha1sum (hex)	b0d6f76af8ad26a7f3a1efe0a089cf4de5d2ef57
schema:url	https://doi.org/10.1016/j.provac.2010.03.009
resource representing a document's title	Analysis of the mechanism by which BALB/c mice having prior immunization with nucleocapsid protein of SARS-CoV develop severe pneumonia after SARS-CoV infection
has PubMed Central identifier	PMC7128161
has PubMed identifier	32288911
schema:publication	Procedia in Vaccinology
resource representing a document's body	covid:b0d6f76af8ad26a7f3a1efe0a089cf4de5d2ef57#body_text
is schema:about of	named entity 'severe acute respiratory syndrome (SARS)' named entity 'nucleocapsid' named entity 'pulmonary' named entity 'severe' named entity 'mice' named entity 'mice' named entity 'SARS-CoV' named entity 'pulmonary' named entity 'LOW' named entity 'LATER' named entity 'SARS' named entity 'CONTRAST' named entity 'PULMONARY' named entity 'SUGGEST' named entity 'STRAIN' named entity 'LOWER' named entity 'LC16M8' named entity 'TH1' named entity 'INVOLVED' named entity 'PNEUMONIA' named entity 'SARS-ASSOCIATED CORONAVIRUS' named entity 'REPORTED' named entity 'DAYS' named entity 'CYTOKINES' named entity 'STRUCTURAL PROTEINS' named entity 'CELLS' named entity 'UNCLEAR' named entity 'MORE THAN' covid:arg/b0d6f76af8ad26a7f3a1efe0a089cf4de5d2ef57 named entity 'PROTEINS' named entity 'TGF' named entity 'PATHOGENESIS' named entity 'TIER' named entity 'INCLUDING' named entity 'PRIOR' named entity 'IL-5' named entity 'THEIR' named entity 'NUCLEOCAPSID' named entity 'NMES' named entity 'SEVERE PNEUMONIA' named entity 'ANTI-INFLAMMATORY CYTOKINE' named entity 'MICE' named entity 'SIGNIFICANT' named entity 'UNDERSTANDING' named entity 'SARS-COV' named entity 'SLIGHT' named entity 'CONTROL' named entity 'UP-REGULATION' named entity 'INFILTRATION' named entity 'TH2' named entity 'SIMILAR' named entity 'HOST IMMUNE RESPONSE' named entity 'PROTEIN ' named entity 'MECHANISM' named entity 'OUR' named entity 'FINDINGS' named entity 'INCREASE' named entity 'CYTOKINE ' named entity 'GROUPS' named entity 'CAUSED BY' named entity 'DOWN-REGULATION' named entity 'PRECISE' named entity 'RESPONSIBLE' named entity 'DETERMINED' named entity 'N PROTEIN' named entity 'RESULTS' named entity 'MEMBRANE' named entity 'STRUCTURAL PROTEIN' named entity 'THESE' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'INFECTED' named entity 'SIMULTANEOUSLY' named entity 'BALB' named entity 'EXPRESSED'

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