About: The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope

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About: The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope
Creator	Bartnik, E Blobel, G Gerace, L Greber, U Senior, A Wozniak, R
Source	PMC
abstract	The glycoprotein gp210 is located in the %22pore membrane,%22 a specialized domain of the nuclear envelope to which the nuclear pore complex (NPC) is anchored. gp210 contains a large cisternal domain, a single transmembrane segment (TM), and a COOH-terminal, 58-amino acid residue cytoplasmic tail (CT) (Wozniak, R. W., E. Bartnik, and G. Blobel. 1989. J. Cell Biol. 108:2083-2092; Greber, U. F., A. Senior, and L. Gerace. 1990. EMBO (Eur. Mol. Biol. Organ.) J. 9:1495-1502). To locate determinants for sorting of gp210 to the pore membrane, we constructed various cDNAs coding for wild-type, mutant, and chimeric gp210, and monitored localization of the expressed protein in 3T3 cells by immunofluorescence microscopy using appropriate antibodies. The large cisternal domain of gp210 (95% of its mass) did not reveal any sorting determinants. Surprisingly, the TM of gp210 is sufficient for sorting to the pore membrane. The CT also contains a sorting determinant, but it is weaker than that of the TM. We propose specific lateral association of the transmembrane helices of two proteins to yield either a gp210 homodimer or a heterodimer of gp210 and another protein. The cytoplasmically oriented tails of these dimers may bind cooperatively to the adjacent NPCs. In addition, we demonstrate that gp210 co-localizes with cytoplasmically dispersed nucleoporins, suggesting a cytoplasmic association of these components.
has issue date	1992-12-02(xsd:dateTime)
bibo:pmid	1281815
has license	cc-by-nc-sa
sha1sum (hex)	b06f31e9139111afe43967edf19c19335b9e79a4
resource representing a document's title	The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope
has PubMed Central identifier	PMC2289754
has PubMed identifier	1281815
schema:publication	J Cell Biol
resource representing a document's body	covid:b06f31e9139111afe43967edf19c19335b9e79a4#body_text
is schema:about of	named entity 'Rockefeller University Press' named entity 'coding' named entity 'EMBO' named entity 'NUCLEAR PORE COMPLEX' named entity 'MONITORED' named entity '3T3 CELLS' named entity 'PORE' named entity 'NUCLEAR ENVELOPE' named entity 'LOCALIZATION' named entity 'AMINO ACID RESIDUE' named entity 'EXPRESSED' named entity 'UNIVERSITY' named entity 'IMMUNOFLUORESCENCE' named entity 'TRANSMEMBRANE' named entity 'ANCHORED' named entity 'CHIMERIC' named entity 'LARGE' named entity 'CONSTRUCTED' named entity 'SINGLE' named entity 'VARIOUS' named entity 'LOCATED IN' named entity 'MEMBRANE' named entity 'GP210' named entity 'CYTOPLASMIC TAIL' named entity 'DOMAIN' named entity 'SPECIALIZED' named entity 'CONTAINS' named entity 'SORTING' named entity 'SEGMENT' named entity 'CODING' named entity 'GLYCOPROTEIN' named entity 'PROTEIN ' named entity 'MUTANT' named entity 'WILD-TYPE' named entity 'DETERMINANTS' named entity 'protein' named entity 'nuclear envelope' named entity 'membrane' named entity 'chimeric' named entity 'gp210' named entity 'NPC' named entity 'sorting' named entity 'nuclear pore complex' named entity 'DNA sequencing' named entity 'antibodies' named entity 'BSA' named entity 'gp210' named entity 'BamHI' named entity 'Immunofluorescence' named entity 'protein' named entity 'gp210' named entity 'nucleotides' named entity 'plasmid' named entity 'in-frame' named entity 'wild-type' named entity 'gp210' named entity 'milk' named entity 'gp210' named entity 'gp210' named entity 'CD8' named entity 'milk' named entity 'cDNA' named entity 'plasma membrane' named entity 'expression vector' named entity 'proteolytic enzymes' named entity 'gp210' named entity 'Golgi' named entity 'cytoplasm' named entity 'integral membrane protein' named entity 'Eppendorf' named entity 'dimer' named entity 'Rochester, NY' named entity 'antibody' named entity 'amino acid residues' named entity 'eDNA' named entity 'antibodies' named entity 'endocytotic' named entity 'CD8'

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