About: Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles
Creator	Prange, Reinhild Lambert, Carsten Thomé, Nicole Kluck, Christoph
Source	Elsevier; Medline; PMC
abstract	Abstract The envelope of hepatitis B virus (HBV), containing the L, M, and S proteins, is essential for virus entry and maturation. For direct visualization of HBV, we determined whether envelope assembly could accommodate the green fluorescent protein (GFP). While the C-terminal addition of GFP to S trans-dominant negatively inhibited empty envelope particle secretion, the N-terminal GFP fusion to S (GFP.S) was co-integrated into the envelope, giving rise to fluorescent particles. Microscopy and topogenesis analyses demonstrated that the proper intracellular distribution and folding of GFP.S, required for particle export were rescued by interprotein interactions with wild-type S. Thereby, a dual location of GFP, inside and outside the envelope, was observed. GFP.S was also efficiently packaged into the viral envelope, and these GFP-tagged virions retained the capacity for attachment to HBV receptor-positive cells in vitro. Together, GFP-tagged virions should be suitable to monitor HBV uptake and egress in live hepatocytes.
has issue date	2004-12-05(xsd:dateTime)
bibo:doi	10.1016/j.virol.2004.09.031
bibo:pmid	15527842
has license	els-covid
sha1sum (hex)	b01451e5d820ef3e258a07f61ff8d68cac7f5af3
schema:url	https://doi.org/10.1016/j.virol.2004.09.031
resource representing a document's title	Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles
has PubMed Central identifier	PMC7111749
has PubMed identifier	15527842
schema:publication	Virology
resource representing a document's body	covid:b01451e5d820ef3e258a07f61ff8d68cac7f5af3#body_text
is schema:about of	named entity 'envelope' named entity 'wild-type' named entity 'folding' covid:arg/b01451e5d820ef3e258a07f61ff8d68cac7f5af3 named entity 'visualization' named entity 'envelope' named entity 'assembly' named entity 'fluorescent protein' named entity 'inhibited' named entity 'GFP' named entity 'For' named entity 'empty' named entity 'receptor' named entity 'virions' named entity 'green fluorescent protein' named entity 'GFP' named entity 'HBV' named entity 'Microscopy' named entity 'GFP' named entity 'hepatocytes' named entity 'green fluorescent protein' named entity 'determined' named entity 'GFP' named entity 'HBV' named entity 'trans' named entity 'GFP' named entity 'Elsevier Inc.' named entity 'GFP' named entity 'HBV' named entity 'HBV' named entity 'hepatitis B virus' named entity 'N-terminal' named entity 'viral envelope' named entity 'virus entry' named entity 'secretion' named entity 'GFP' named entity 'particle export' named entity 'GFP' named entity 'intracellular' named entity 'virions' named entity 'GFP' named entity 'wild-type' named entity 'fluorescent' named entity 'GFP' named entity 'hepatitis B virus' named entity 'fusion' named entity 'HBV' named entity 'All rights reserved' named entity 'virus' named entity 'packaged' named entity 'GFP' named entity 'HBV' named entity 'rise' named entity 'monitor' named entity 'export' named entity 'virions' named entity 'particle' named entity 'viral envelope' named entity 'direct' named entity 'attachment' named entity 'fluorescent' named entity 'retained' named entity 'hepatocytes' named entity 'particles' named entity 'While' named entity 'envelope' named entity 'N-terminal' named entity 'envelope' named entity 'distribution' named entity 'particle' named entity 'HBV' named entity 'incorporation' named entity 'Functional' named entity 'HBV' named entity 'intracellular' named entity 'rescued' named entity 'GFP' named entity 'secretion'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software