About: Insect Contribution to Horizontal Transmission of Reticuloendotheliosis virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Insect Contribution to Horizontal Transmission of Reticuloendotheliosis virus
Creator	Davidson, Irit Braverman, Yehuda
topic	covid:afd2d43ec2ecb7358f841b6304cbb011fd625928#this
source	Medline; PMC
abstract	The involvement of insects in Reticuloendotheliosis virus (REV) transmission was examined by testing insects trapped at commercial farms and by controlled feeding experiments using mosquitoes, Culex pipiens L. and house flies, Musca domestica L. We established sensitive methods of REV detection, including reverse transcription-polymerase chain reaction (PCR) for REV-LTR and REV-gag genes, REV antigenemia measurements by enzyme-linked immunosorbent assay, and virus isolation in tissue cultures. A variety of blood-sucking species of insects were trapped at farms with infected poultry and tested, but none were positive. To rule out the possibility of PCR inhibition by insect RNA, spiking experiments were conducted and no interference was observed. Because Cx. pipiens mosquitoes were trapped frequently at farms, we performed feeding experiments with mosquito females fed on a REV-containing tissue culture medium and chicken blood mixture. Virus was detected in the mosquitoes up to 5 h postfeeding, compared with 96 h in the feeding mixture, indicating that Cx. pipiens can only harbor REV for a short period. House flies were suspected to be involved in the virus transmission because they frequently were trapped on positive farms. In contrast to mosquitoes, REV was harbored within the house fly digestive tract for up to 72 h and could infect chickens, as demonstrated by seroconversion and by detection of viral gag-sequence in the cloaca. The current study is supportive for the role of house flies as a mechanical vector of REV among poultry.
has issue date	2005-03-01(xsd:dateTime)
bibo:doi	10.1093/jmedent/42.2.128
bibo:pmid	15799521
has license	no-cc
sha1sum (hex)	afd2d43ec2ecb7358f841b6304cbb011fd625928
schema:url	https://doi.org/10.1093/jmedent/42.2.128
resource representing a document's title	Insect Contribution to Horizontal Transmission of Reticuloendotheliosis virus
has PubMed Central identifier	PMC7107493
has PubMed identifier	15799521
schema:publication	J Med Entomol
resource representing a document's body	covid:afd2d43ec2ecb7358f841b6304cbb011fd625928#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/afd2d43ec2ecb7358f841b6304cbb011fd625928
is schema:about of	named entity 'seroconversion' named entity 'involved' named entity 'infect' named entity 'transmission' named entity 'reverse' named entity 'sensitive' named entity 'mosquitoes' named entity 'measurements' named entity 'short' named entity 'CONTRIBUTION' named entity 'RETICULOENDOTHELIOSIS VIRUS' named entity 'INVOLVEMENT' named entity 'ESTABLISHED' named entity 'TISSUE CULTURE' named entity 'MIXTURE' named entity 'FED' named entity 'CHICKENS' named entity 'PERFORMED' named entity 'SPIKING' named entity 'METHODS' named entity 'SUCKING' named entity 'TRANSMISSION' named entity 'COMMERCIAL' named entity 'REVERSE TRANSCRIPTION' named entity 'CONTROLLED' named entity 'VIRUS' named entity 'TESTING' named entity 'INSECTS' named entity 'POULTRY' named entity 'EXAMINED' named entity 'FEMALES' named entity 'VARIETY' named entity 'CONTRAST' named entity 'POSITIVE' named entity 'SUSPECTED' named entity 'CHICKEN' named entity 'EXPERIMENTS' named entity 'STUDY' named entity 'FARMS' named entity 'DIGESTIVE TRACT' named entity 'MUSCA DOMESTICA' named entity 'CONTAINING' named entity 'SEROCONVERSION' named entity 'SHORT PERIOD' named entity 'UP TO' named entity 'INVOLVED' named entity 'REV' named entity 'INDICATING' named entity 'INTERFERENCE' named entity 'INSECT' named entity 'SUPPORTIVE' named entity 'RULE OUT' named entity 'OBSERVED' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'PCR' named entity 'BLOOD' named entity 'INSECT' named entity 'CULTURE MEDIUM' named entity 'SENSITIVE' named entity 'POLYMERASE CHAIN REACTION' named entity 'MOSQUITOES' named entity 'INFECT' named entity 'POSSIBILITY OF' named entity 'HOUSE' named entity 'INFECTED' named entity 'TRAPPED' named entity 'GAG GENES' named entity 'BUT' named entity 'FREQUENTLY' named entity 'DETECTION' named entity 'GAG' named entity 'DETECTED' named entity 'LTR' named entity 'SPECIES' named entity 'CULEX PIPIENS' named entity 'RETICULOENDOTHELIOSIS VIRUS' named entity 'INCLUDING' named entity 'ROLE'

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